NDEA

N-nitrosodiethylamine genotoxicity evaluation: a cytochrome P450 induction study in rat hepatocytes

C. A. F. Aiub, Gadermaier, G., Silva, I. O., Felzenszwalb, I., Pinto, L. F. R., Ferreira, F., and Eckl, P., N-nitrosodiethylamine genotoxicity evaluation: a cytochrome P450 induction study in rat hepatocytes, vol. 10, pp. 2340-2348, 2011.

In rats, N-nitrosodiethylamine (NDEA) induces tumors mainly in the liver. This could be because various enzymes are responsible for the metabolic activation of NDEA, besides the hepatic NDEA metabolizing enzyme, CYP2E1. We examined NDEA genotoxicity and cytotoxicity in primary cultures of female rat hepatocytes; we also looked at how it affected CYP mRNA expression. Single incubation with 0.9% NaCl resulted in a mean of 0.2% apoptotic cells, which doubled with 105 μg NDEA/mL. The frequency of necrosis with NDEA treatment was also doubled.

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