nested-polymerase chain reaction

Molecular basis for porcine parvovirus detection in dead fetuses

V. H. G. Wolf, Menossi, M., Mourão, G. B., and Gatti, M. S. V., Molecular basis for porcine parvovirus detection in dead fetuses, vol. 7, pp. 509-517, 2008.

Reproductive failures are still common grounds for complaint by commercial swine producers. Porcine parvovirus (PPV) is associated with different clinical reproductive signs. The aim of the present study was to investigate PPV fetal infection at swine farms having ongoing reproductive performance problems. The presence of virus in fetal tissues was determined by nested-polymerase chain reaction assay directed to the conserved NS1 gene of PPV in aborted fetuses, mummies and stillborns. Fetuses show a high frequency of PPV infection (96.4%; n = 28).

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