Novel gene

Molecular cloning and characterization of a novel chicken gene named grni

Y. Hua, Hui, D. Y., Rong, Z. H., and Cheng, T., Molecular cloning and characterization of a novel chicken gene named grni, vol. 13, pp. 386-395, 2014.

The full-length cDNA sequence of a novel expressed sequence tag (GenBank accession No. HQ184338) that was differentially expressed during Newcastle disease virus (NDV) infection in chickens was cloned from the chicken spleen by a rapid amplification of cDNA ends assay. This gene was further analyzed using bioinformatic methods and named grni. The full-length cDNA sequence was 1698 bp without introns, locating between 104,691,934 and 104,693,618 in galGal4 on chromosome 2. The open reading frame (ORF) contained 261 bp and encoded a deduced protein of 86 amino acid residues.

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