Oligopeptide permease

Detection of soluble expression and in vivo interactions of the inner membrane protein OppC using green fluorescent protein

Q. J. Xiang, Zhai, J. F., Zhang, M., and Zhang, B., Detection of soluble expression and in vivo interactions of the inner membrane protein OppC using green fluorescent protein, vol. 14, pp. 17834-17846, 2015.

In this study, the in vivo interaction system of oligopeptide permease (Opp) proteins was analyzed, and a high expression system of inner membrane protein OppC was constructed by flexible usage of the green fluorescent protein (GFP). The Escherichia coli OppC gene, which encodes a transmembrane component of oligopeptide transporter, was cloned into different vectors. Recombinant plasmids were transformed into different E. coli strains, and the expression conditions were optimized.

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