Optical density

Source of bacterial RNA in chronic otitis media with effusion

P. - Z. Li, Cheng, L., and Qiu, M. - L., Source of bacterial RNA in chronic otitis media with effusion, vol. 13, pp. 6093-6098, 2014.

The purpose of this study was to investigate whether the bacterial RNA detected by polymerase chain reaction (PCR) and reverse transcription (RT)-PCR methods in middle ear effusion (MEE) for pediatric chronic otitis media with effusion (OME) originated from live bacteria. Degradation of RNA was observed by spectroscopic analysis; we also investigated the effect of MEE on the digestive activity of RNase. The optical density of RNA solution was stable within 3 h. MEE could not degrade the RNA, while RNase could rapidly digest the RNA.

Subscribe to Optical density