Optimization of SCoT-PCR reaction system in Dactylis glomerata by orthogonal design
, “Optimization of SCoT-PCR reaction system in Dactylis glomerata by orthogonal design”, vol. 14, pp. 3052-3061, 2015.
The effects of 5 factors (template DNA, Mg2+, dNTPs, Taq DNA polymerase, and primer) on the polymerase chain reaction (PCR) were investigated to optimize the start codon targeted polymorphism (SCoT)-PCR system of Dactylis glomerata L., using an orthogonal design L16 (45). A suitable SCoT-PCR system for D. glomerata was established; the 20 μL reaction volume contained 3.0 mM Mg2+, 0.2 mM dNTPs, 1.0 U Taq DNA polymerase, 0.2 μM primer, 20 ng template DNA, and 2 μL 10X buffer.
