Orchid

A putative miR172-targeted CeAPETALA2-like gene is involved in floral patterning regulation of the orchid Cymbidium ensifolium

F. X. Yang, Zhu, G. F., Wang, Z., Liu, H. L., and Huang, D., “A putative miR172-targeted CeAPETALA2-like gene is involved in floral patterning regulation of the orchid Cymbidium ensifolium”, vol. 14, pp. 12049-12061, 2015.

APETALA2 plays critical roles in establishing meristem and organ identity during plant floral development. In this study, we obtained a CeAP2-like gene by using the mRNA differential display technique to analyze the wild type and a multitepal mutant of the orchid Cymbidium ensifolium. The full-length cDNA encoding the CeAP2-like transcription factor shows significant similarity to the cDNA of AP2 from Erycina pusilla and contains nucleotides complementary to miR172.

Evaluation of genetic variability in in vitro sodium azide-induced Dendrobium ‘Earsakul’ mutants

A. Wannajindaporn, Poolsawat, O., Chaowiset, W., and Tantasawat, P. A., “Evaluation of genetic variability in in vitro sodium azide-induced Dendrobium ‘Earsakul’ mutants”, vol. 13, pp. 5333-5342, 2014.

In vitro mutagenesis of Dendrobium ‘Earsakul’ was carried out by incubating the protocorm-like bodies in 0-5 mM sodium azide for 1 h. Twenty-eight putative mutants were evaluated for genetic variability compared to untreated control plants using inter-simple sequence repeat (ISSR) analysis. Polymorphic fragments were produced by 9 of 12 ISSR primers. A total of 173 amplified ISSR fragments varying in size from 140 to 5000 bp were obtained, 39 of which were polymorphic (22.5%).

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