OSP-1

Transcriptional activity of an ovarian-specific promoter from rat in dairy goat granulosa cells

H. - Y. Liu, Yang, M. - M., Cui, Y. - H., Ma, T., Liu, X. - Q., Bai, L., Xiao, W. - P., Zhao, H. - B., Peng, J. - Y., Hu, X. - B., and Cao, B. - Y., Transcriptional activity of an ovarian-specific promoter from rat in dairy goat granulosa cells, vol. 12, pp. 127-135, 2013.

Ovarian-specific promoter 1 (OSP-1) is a retrovirus-like element isolated from the complementary DNA library of rat that has been thought to be specifically expressed in ovary. To exploit this promoter in dairy goat ovary granulosa cells (GCs), OSP-1 from rat was used to construct the reporter vector pOSP-1-EGFP, in which egfp coding for enhanced green fluorescent protein (EGFP) was used as a reporter to examine the activity of OSP-1 in GCs. EGFP was successfully expressed in dairy goat GCs transfected with pOSP-1-EGFP.

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