Osteogenic differentiation

Identification of long non-coding RNA involved in osteogenic differentiation from mesenchymal stem cells using RNA-Seq data

W. Q. Song, Gu, W. Q., Qian, Y. B., Ma, X., Mao, Y. J., and Liu, W. J., Identification of long non-coding RNA involved in osteogenic differentiation from mesenchymal stem cells using RNA-Seq data, vol. 14, pp. 18268-18279, 2015.

The aim of this study was to identify long non-coding RNA (lncRNA) associated with osteogenic differentiation from mesenchymal stem cells (MSCs) using high-throughput RNA sequencing (RNA-Seq) data. RNA-Seq dataset was obtained from the European Bioinformatics Institute (accession No. PRJEB4496), including two replicates each for immortalized mesenchymal stem cells iMSC#3 cultured in growth medium (GM) and differentiation medium (DM) for 28 days. The clean reads were aligned to a hg19 reference genome by Tophat and assembled by Cufflinks to identify the known and novel transcripts.

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