Overexpression

Cloning and characterization of peanut allene oxide cyclase gene involved in salt-stressed responses

H. H. Liu, Wang, Y. G., Wang, S. P., and Li, H. J., Cloning and characterization of peanut allene oxide cyclase gene involved in salt-stressed responses, vol. 14, pp. 2331-2340, 2015.

In this study, the full-length cDNA encoding allene oxide cyclase (AhAOC) was isolated from peanut (Arachis hypogaea L.). The deduced amino acid sequence of AhAOC showed high homology with other plant AOCs. The transcript of AhAOC was found to be abundantly expressed in roots. Expression analysis demonstrated that AhAOC was induced by abscisic acid, methyl-jasmonic acid, salicylic acid, salinity, polyethylene glycol, and cold stresses, particularly by high salinity.

Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice

Z. W. Liu, Fan, H. L., Liu, X. F., Ding, X. B., Wang, T., Sui, G. N., Li, G. P., and Guo, H., Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice, vol. 14, pp. 2742-2749, 2015.

Adipocyte fatty acid-binding protein (A-FABP), the most abundant FABP in adipocytes, controls fatty acid uptake, transport, and metabolism in fat cells. We constructed a transgenic mice model that overexpressed the cattle A-FABP gene to investigate the relationship between A-FABP expression and intermuscular fat deposition. There was no significant difference in body weight and serum biochemical indexes between transgenic and wild-type mice.

Comparative analysis and molecular characterization of genomic sequences and proteins of FABP4 and FABP5 from the giant panda (Ailuropoda melanoleuca)

B. Song, Hou, Y. L., Ding, X., Wang, T., Wang, F., Zhong, J. C., Xu, T., Zhong, J., Hou, W. R., and Shuai, S. R., Comparative analysis and molecular characterization of genomic sequences and proteins of FABP4 and FABP5 from the giant panda (Ailuropoda melanoleuca), vol. 13, pp. 992-1004, 2014.

Fatty acid binding proteins (FABPs) are a family of small, highly conserved cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. In this study, cDNA and genomic sequences of FABP4 and FABP5 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using reverse transcription polymerase chain reaction (RT-PCR) technology and touchdown-PCR.

cDNA, genomic sequence cloning and overexpression of giant panda (Ailuropoda melanoleuca) mitochondrial ATP synthase ATP5G1

W. - R. Hou, Hou, Y. - L., Ding, X., and Wang, T., cDNA, genomic sequence cloning and overexpression of giant panda (Ailuropoda melanoleuca) mitochondrial ATP synthase ATP5G1, vol. 11, pp. 3164-3174, 2012.

The ATP5G1 gene is one of the three genes that encode mitochondrial ATP synthase subunit c of the proton channel. We cloned the cDNA and determined the genomic sequence of the ATP5G1 gene from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively. The cloned cDNA fragment contains an open reading frame of 411 bp encoding 136 amino acids; the length of the genomic sequence is of 1838 bp, containing three exons and two introns.

Overexpression, purification, and pharmacologic evaluation of anticancer activity of ribosomal protein L24 from the giant panda (Ailuropoda melanoleuca)

Y. L. Hou, Ding, X., Hou, W., Song, B., Wang, T., Wang, F., Li, J., Zhong, J., Xu, T., Ma, B. X., Zhu, H. Q., Li, J. H., and Zhong, J. C., Overexpression, purification, and pharmacologic evaluation of anticancer activity of ribosomal protein L24 from the giant panda (Ailuropoda melanoleuca), vol. 12, pp. 4735-4750, 2013.

The ribosomal protein L24 (RPL24) belongs to the L24E family of ribosomal proteins and is located in the cytoplasm. The purpose of this study was to investigate the structure and anti-cancer function of RPL24 of the giant panda (Ailuropoda melanoleuca). The complementary DNA of RPL24 was cloned successfully using reverse transcription-polymerase chain reaction technology. We constructed a recombinant expression vector containing RPL24 complementary DNA and overexpressed it in Escherichia coli using pET28a plasmids.

Oct4 and Sox2 overexpression improves the proliferation and differentiation of bone mesenchymal stem cells in Xiaomeishan porcine

Y. X. Fan, Gu, C. H., Zhang, Y. L., Zhong, B. S., Wang, L. Z., Zhou, Z. R., Wang, Z. Y., Jia, R. X., and Wang, F., Oct4 and Sox2 overexpression improves the proliferation and differentiation of bone mesenchymal stem cells in Xiaomeishan porcine, vol. 12, pp. 6067-6079, 2013.

Mesenchymal stem cells derived from bone marrow (BMSCs) are a population of self-renewing multipotent cells that are capable of differentiating into various cellular lineages, and are widely employed in tissue engineering and cell therapy. Recently, clinical research involving BMSCs has become increasingly popular. In order to conduct appropriate research, it is first necessary to amplify large amounts of functional BMSCs in vitro. However, after several passages of expanding in vitro, the proliferation and differentiation potential of BMSCs gradually decline.

Functions of microRNA in response to cocaine stimulation

L. - F. Xu, Wang, J., Lv, F. B., and Song, Q., Functions of microRNA in response to cocaine stimulation, vol. 12, pp. 6160-6167, 2013.

MicroRNAs (miRNAs) are a type of non-protein-coding single-stranded RNA, which are typically 20-25 nt in length. miRNAs play important roles in various biological processes, including development, cell proliferation, differentiation, and apoptosis. We aimed to detect the miRNA response to cocaine stimulations and their target genes. Using the miRNA expression data GSE21901 downloaded from the Gene Expression Omnibus database, we screened out the differentially expressed miRNA after short-term (1 h) and longer-term (6 h) cocaine stimulations based on the fold change >1.2.

Overexpression of HSP27 in cultured human aortic smooth muscular cells reduces apoptosis induced by low-frequency and low-energy ultrasound by inhibition of an intrinsic pathway

B. Zhang, Zhou, H. S., Cheng, Q., Lei, L., and Hu, B., Overexpression of HSP27 in cultured human aortic smooth muscular cells reduces apoptosis induced by low-frequency and low-energy ultrasound by inhibition of an intrinsic pathway, vol. 12, pp. 6588-6601, 2013.

We investigated in vitro the effect of low-frequency and low-energy ultrasound (LFLEU) on apoptosis of an overexpressed HSP27 human aortic smooth muscle cell (HASMC) line. A frequency of 42.6 kHz was used in all experiments. HASMC were exposed to ultrasound and cell viability was evaluated by MTT reduction. Overexpressed HSP27-HASMC was constructed on a pcDNA3.1 vector. Apoptosis was determined 24 h after treatment by flow cytometry; gene display was evaluated with Affimax chips, and HSP27 mRNA and protein expression levels were measured by RT-PCR and Western blotting.

Giant panda ribosomal protein S14: cDNA, genomic sequence cloning, sequence analysis, and overexpression

G. - F. Wu, Hou, Y. - L., Hou, W. - R., Song, Y., and Zhang, T., Giant panda ribosomal protein S14: cDNA, genomic sequence cloning, sequence analysis, and overexpression, vol. 9, pp. 2004-2015, 2010.

RPS14 is a component of the 40S ribosomal subunit encoded by the RPS14 gene and is required for its maturation. The cDNA and the genomic sequence of RPS14 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively; they were both sequenced and analyzed. The length of the cloned cDNA fragment was 492 bp; it contained an open-reading frame of 456 bp, encoding 151 amino acids. The length of the genomic sequence is 3421 bp; it contains four exons and three introns.

cDNA, genomic sequence cloning and overexpression of the ribosomal protein S13 gene in the giant panda (Ailuropoda melanoleuca)

Y. Song, Hou, Y. - L., Hou, W. - R., Wu, G. - F., and Zhang, T., cDNA, genomic sequence cloning and overexpression of the ribosomal protein S13 gene in the giant panda (Ailuropoda melanoleuca), vol. 10, pp. 121-132, 2011.

The cDNA and the genomic sequence of ribosomal protein S13 (RPS13) of the giant panda (Ailuropoda melanoleuca) was cloned using reverse transcription-polymerase chain reaction (RT-PCR) and touchdown-PCR, respectively. These two sequences were sequenced and analyzed, and the cDNA of the RPS13 gene was overexpressed in Escherichia coli BL21. We compared the nucleotide sequences of the coding region and the amino acid sequences with those of seven other mammalian species retrieved from GenBank.

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