Oxidative stress

Regulation of expression of sodA and msrA genes of Corynebacterium glutamicum in response to oxidative and radiative stress

H. M. El Shafey and Ghanem, S., Regulation of expression of sodA and msrA genes of Corynebacterium glutamicum in response to oxidative and radiative stress, vol. 14, pp. 2104-2117, 2015.

Promoters of genes encoding superoxide dismutase (sodA) and peptide methionine sulfoxide reductase (msrA) from Cory­nebacterium glutamicum were cloned and sequenced. Promoter region analysis of sodA-msrA was unable to identify putative sites of fixed eventual regulators except for possible sites of fixed OxyR and integra­tion host factor. A study of the regulation of these genes was performed using the lacZ gene of Escherichia coli as a reporter placed under the control of sequences downstream of sodA and msrA.

Protective effect of proanthocyanidin against oxidative ovarian damage induced by 3-nitropropionic acid in mice

J. Q. Zhang, Xing, B. S., Zhu, C. C., Shen, M., Yu, F. X., and Liu, H. L., Protective effect of proanthocyanidin against oxidative ovarian damage induced by 3-nitropropionic acid in mice, vol. 14, pp. 2484-2494, 2015.

Oxidative stress, which poses a threat to reproductive health, causes many serious female reproductive diseases. In this study, we investigated whether proanthocyanidins (PC) have a protective effect against oxidative stress-induced ovarian damage. Forty female ICR mice were randomized into 4 groups: a control group, a control plus PC group, a 3-nitropropionic acid (3-NPA) group, and a 3-NPA plus PC group. An ovarian oxidative stress model induced by 3-NPA was constructed using female ICR mice.

Influence of cyclophilin D protein expression level on endothelial cell oxidative damage resistance

J. Z. Peng, Xue, L., Chen, J., Chen, B. S., and Yang, Y. Q., Influence of cyclophilin D protein expression level on endothelial cell oxidative damage resistance, vol. 14, pp. 4258-4268, 2015.

We examined the influence of cyclophilin-D (CypD) protein expression level on endothelial cell oxidative damage resistance. A model of CypD protein expression or high expression in endothelial cells was established through gene silencing or cloning. The comparable groups were normal endothelial cells cultured in phosphate-buffered solution in liquid handling cells containing 500 mM H2O2 for 90 or 120 min, and then the medium was replaced with common nutrient solution and cultured again for 24 h.

Ascorbate peroxidase from Jatropha curcas enhances salt tolerance in transgenic Arabidopsis

Y. Chen, Cai, J., .X.Yang, F., Zhou, B., and Zhou, L. R., Ascorbate peroxidase from Jatropha curcas enhances salt tolerance in transgenic Arabidopsis, vol. 14, pp. 4879-4889, 2015.

Ascorbate peroxidase (APX) plays a central role in the ascorbate-glutathione cycle and is a key enzyme in cellular H2O2 me­tabolism. It includes a family of isoenzymes with different character­istics, which are identified in many higher plants. In the present study, we isolated the APX gene from Jatropha curcas L, which is similar with other previously characterized APXs as revealed by alignment and phylogenetic analysis of its deduced amino acid sequence.

Evaluation of glutathione peroxidase and superoxide dismutase enzyme polymorphisms in celiac disease patients

M. Katar, Ozugurlu, A. F., Ozyurt, H., and Benli, I., Evaluation of glutathione peroxidase and superoxide dismutase enzyme polymorphisms in celiac disease patients, vol. 13, pp. 1030-1037, 2014.

Celiac disease (CD) is a multifactorial, inflammatory small bowel disorder characterized by nutrient malabsorption resulting from mucosal damage, the latter induced by cereal products like barley, oat, and wheat. Oxidative stress has previously been reported to play an important role in the pathogenesis of CD.

Genetic polymorphisms of paraoxonase1 192 and glutathione peroxidase1 197 enzymes in familial Mediterranean fever

F. Öktem, Anıl, H., Sütcü, R., and Kuybulu, A. E., Genetic polymorphisms of paraoxonase1 192 and glutathione peroxidase1 197 enzymes in familial Mediterranean fever, vol. 13, pp. 3292-3300, 2014.

Familial Mediterranean fever (FMF) is an autosomal recessive disorder and is the most frequent of the periodic febrile inflammatory syndromes. The pathogenesis of the disease is not completely understood, even though the FMF gene has been identified. Oxidative stress and inflammation may play a role in the pathogenesis of FMF. We investigated gene polymorphisms of the antioxidative enzymes, glutathione peroxidase (GPX) and paraoxonase (PON) in FMF patients, and possible associations with FMF pathogenesis.

A potential protective role for thiamine in glucose-driven oxidative stress

B. Palabiyik, F. Ghods, J., and E. Ucar, O., A potential protective role for thiamine in glucose-driven oxidative stress, vol. 13, pp. 5582-5593, 2014.

The relationship between glucose repression and the oxidative stress response was investigated in Schizosaccharomyces pombe wild type cells (972h-) and glucose repression resistant mutant type cells (ird11). We aimed to reveal the mechanism of simultaneous resistance to glucose repression and oxidative stress in ird11 mutants. Compared to the wild type, the expression of the sty1 gene was not altered in the ird11 mutant under normal growth conditions, but decreased after exposure to H2O2.

DNA and RNA polymerase activity in a Moniliophthora perniciosa mitochondrial plasmid and self-defense against oxidative stress

B. S. Andrade, Villela-Dias, C., Gomes, D. S., Micheli, F., and Góes-Neto, A., DNA and RNA polymerase activity in a Moniliophthora perniciosa mitochondrial plasmid and self-defense against oxidative stress, vol. 12, pp. 1944-1950, 2013.

Moniliophthora perniciosa (Stahel) Aime and Phillips-Mora is a hemibiotrophic basidiomycete (Agaricales, Tricholomataceae) that causes witches’ broom disease in cocoa (Theobroma cacao L.). This pathogen carries a stable integrated invertron-type linear plasmid in its mi­tochondrial genome that encodes viral-like DNA and RNA polymerases re­lated to fungal senescence and longevity. After culturing the fungus and ob­taining its various stages of development in triplicate, we carried out total RNA extraction and subsequent complementary DNA synthesis.

Effects of Thai black sticky rice extract on oxidative stress and lipid metabolism gene expression in HepG2 cells

W. Sangkitikomol, Tencomnao, T., and Rocejanasaroj, A., Effects of Thai black sticky rice extract on oxidative stress and lipid metabolism gene expression in HepG2 cells, vol. 9, pp. 2086-2095, 2010.

Anthocyanins, which are found in some food, including Thai black sticky rice, are reported to have health-promoting properties. Oxidative stress plays a major role in the pathogenesis of many degenerative diseases induced by free radicals, such as cardiovascular disease, stroke and cancer. We evaluated the anthocyanin-rich extract (ARE) from Thai black sticky rice for antioxidative and antihyperlipidemic effects on HepG2 cells. Cell viability was investigated with the neutral red assay and the MTT assay, and oxidative stress was determined by the DCFH-DA assay.

Ala-9Val polymorphism of Mn-SOD gene in sickle cell anemia

S. Sogut, Yonden, Z., Kaya, H., Oktar, S., Tutanc, M., Yilmaz, H. R., Yigit, A., Ozcelik, N., and Gali, E., Ala-9Val polymorphism of Mn-SOD gene in sickle cell anemia, vol. 10, pp. 828-833, 2011.

Oxidative stress may be contributory to the pathophy­siology of the abnormalities that underlie the clinical course of sickle cell anemia. We looked for a possible genetic association between the functional polymorphism Ala-9Val in the human Mn-SOD gene and sickle cell anemia. One hundred and twenty-seven patients with sickle cell anemia and 127 healthy controls were recruited into the study.

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