Passion flower

Improvements in cytological preparations for fluorescent in situ hybridization in Passiflora

M. M. Souza, Urdampilleta, J. D., and Forni-Martins, E. R., Improvements in cytological preparations for fluorescent in situ hybridization in Passiflora, vol. 9, pp. 2148-2155, 2010.

Cytological preparations for the fluorescent in situ hybridization (FISH) technique require cytoplasm-free metaphases, with well-spread chromosomes, for the localization of DNA sequences and chromosome mapping. We tested various procedures for FISH analysis of Passiflora cacaoensis, P. gardneri and hybrid F1 progeny of P. gardneri x P. gibertii. Two treatments with four enzymes and three incubation times were compared. The material was treated with 1.0 M HCl before enzymatic digestion.

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