PCR

Study of the genetic diversity and structure of a natural population of Nectandra megapotamica (Spreng.) Mez. using RAPD markers

L. S. Costa, Reiniger, L. R. S., Heinzmann, B. M., Amaral, L. P., and Serrote, C. M. L., Study of the genetic diversity and structure of a natural population of Nectandra megapotamica (Spreng.) Mez. using RAPD markers, vol. 14, pp. 18407-18413, 2015.

Nectandra megapotamica (Spreng.) Mez. is a tree species that naturally occurs in the Atlantic Forest, Brazil. The aim of this study was to evaluate the genetic diversity and structure of a natural population of 12 N. megapotamica individuals using random amplified polymorphic DNA markers. Eleven primers were used in this study, producing 81 bands, of which 98.99% were polymorphic. Analysis using STRUCTURE defined three different clusters (K = 3), results that were consistent with those of principal coordinates analysis.

Detection of Toxoplasma gondii DNA in naturally infected sheep’s milk

deSantana D. Rocha, Moura, R. Lde Sousa, Maciel, B. M., Guimarães, L. A., O’dwyer, H. N. S., Munhoz, A. D., and Albuquerque, G. R., Detection of Toxoplasma gondii DNA in naturally infected sheep’s milk, vol. 14, pp. 8658-8662, 2015.

The objective of this study was to verify whether Toxoplasma gondii is excreted in the milk of naturally infected sheep. In order to accomplish this, 275 lactating ewes were used; these were bred extensively in 17 estates distributed across nine cities. Polymerase chain reaction amplification was used to detect T. gondii DNA in milk samples, and the indirect immunofluorescence test was employed for the detection of anti-T. gondii IgG antibodies in the sera, with a cut-off value of 1:64.

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