PCR-DGGE

Evaluation of a modified sampling method for molecular analysis of air microflora

T. Lech and Ziembinska-Buczynska, A., Evaluation of a modified sampling method for molecular analysis of air microflora, vol. 14, pp. 3200-3208, 2015.

A serious issue concerning the durability of economically important materials for humans related to cultural heritage is the process of biodeterioration. As a result of this phenomenon, priceless works of art, documents, and old prints have undergone a process of decomposition caused by microorganisms. Therefore, it is important to constantly monitor the presence and diversity of microorganisms in exposition rooms and storage areas of historical objects.

Identification of actinomycete communities in Antarctic soil from Barrientos Island using PCR-denaturing gradient gel electrophoresis

L. Learn-Han, Yoke-Kqueen, C., Shiran, M. S., Vui-Ling, C. M. W., Nurul-Syakima, A. M., Son, R., and Andrade, H. M., Identification of actinomycete communities in Antarctic soil from Barrientos Island using PCR-denaturing gradient gel electrophoresis, vol. 11, pp. 277-291, 2012.

The diversity of specific bacteria taxa, such as the actinomycetes, has not been reported from the Antarctic island of Barrientos. The diversity of actinomycetes was estimated with two different strategies that use PCR-denaturing gradient gel electrophoresis. First, a PCR was applied, using a group-specific primer that allows selective amplification of actinomycete sequences. Second, a nested-PCR approach was used that allows the estimation of the relative abundance of actinomycetes within the bacterial community.

Detection by denaturing gradient gel electrophoresis of ammonia-oxidizing bacteria in microcosms of crude oil-contaminated mangrove sediments

A. C. Fdos Santos, Marques, E. L. S., Gross, E., Souza, S. S., Dias, J. C. T., Brendel, M., and Rezende, R. P., Detection by denaturing gradient gel electrophoresis of ammonia-oxidizing bacteria in microcosms of crude oil-contaminated mangrove sediments, vol. 11, pp. 190-201, 2012.

Currently, the effect of crude oil on ammonia-oxidizing bacterium communities from mangrove sediments is little understood. We studied the diversity of ammonia-oxidizing bacteria in mangrove microcosm experiments using mangrove sediments contaminated with 0.1, 0.5, 1, 2, and 5% crude oil as well as non-contaminated control and landfarm soil from near an oil refinery in Camamu Bay in Bahia, Brazil. The evolution of CO2 production in all crude oil-contaminated microcosms showed potential for mineralization.

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