PCR-RFLP

Polymorphism in the melatonin receptor gene in buffalo populations of the Brazilian Amazon

E. B. Machado, Souza, B. B., Guimarães, R. C., Azevedo, J. S. N., Gonçalves, E. C., Ribeiro, H. F. L., Filho, S. T. Rolim, E. Filho, S., Machado, E. B., Souza, B. B., Guimarães, R. C., Azevedo, J. S. N., Gonçalves, E. C., Ribeiro, H. F. L., Filho, S. T. Rolim, and E. Filho, S., Polymorphism in the melatonin receptor gene in buffalo populations of the Brazilian Amazon, vol. 15, p. -, 2016.

Buffalo farming in Brazil is increasing, as is the challenge of identifying molecular markers that will improve productivity. Therefore, the aim of this study was to analyze single nucleotide polymorphisms of the receptor gene for the hormone melatonin in buffaloes from northern Brazil by polymerase chain reactions (PCRs) and restriction fragment length polymorphism assays. The PCR products exhibited a cutting point for HpaI at the 318th position of the gene, indicating a transition substitution (T↔C).

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