PCR-RFLP

A novel polymorphism of the MYPN gene and its association with meat quality traits in Bos taurus

Y. Jiao, Zan, L. S., Liu, Y. F., Wang, H. B., and Guo, B. L., A novel polymorphism of the MYPN gene and its association with meat quality traits in Bos taurus, vol. 9, pp. 1751-1758, 2010.

Myopalladin (MYPN) is a multifunctional protein that maintains sarcomeric integrity and regulates Z-line structure. It is an important candidate gene for meat quality selection through marker-assisted selection. Using PCR-RFLP technology, we discovered a single-nucleotide polymorphism (SNP) (A1795G in exon 9) of the MYPN gene. Allele frequencies of this SNP were investigated and evaluated by the χ2 test in 660 cattle populations in China; only the Nanyang population was not in Hardy-Weinberg equilibrium.

Influence of the halothane gene (HAL) on pork quality in two commercial crossbreeds

A. C. P. Silveira, Freitas, P. F. A., César, A. S. M., César, A. S. M., Antunes, R. C., Guimarães, E. C., Batista, D. F. A., and Torido, L. C., Influence of the halothane gene (HAL) on pork quality in two commercial crossbreeds, vol. 10, pp. 1479-1489, 2011.

We evaluated the effect of the halothane (HAL) gene on the quality of pork in domestic pigs. Half-carcasses from two different commercial pig (Sus domestica) crossbreeds were analyzed, 46 of which were homozygous dominant (HALNN) and 69 of which were heterozygous (HALNn) for the halothane gene. The measures included backfat thickness, lean meat percentage, carcass weight, pH 24 h after slaughtering, color, and drip loss; DNA was extracted from the haunch muscle.

AGPAT6 polymorphism and its association with milk traits of dairy goats

C. He, Wang, C., Chang, Z. H., Guo, B. L., Li, R., Yue, X. P., Lan, X. Y., Chen, H., and Lei, C. Z., AGPAT6 polymorphism and its association with milk traits of dairy goats, vol. 10, pp. 2747-2756, 2011.

As one of the eight members in the 1-acylglycerol-3-phosphate-O-acyltransferase (AGPATs) family, AGPAT6 is a crucial enzyme for the biosynthesis of glycerolipids and triacylglycerol in eukaryotes, as well as catalyzing the conversion from lysophosphatidic acid to phosphatidic acid. AGPAT6 can be considered as a candidate gene for regulating milk composition. DNA sequencing and PCR-RFLP methods were applied to detect genetic variation in the AGPAT6 gene in 549 Chinese dairy goats.

Lack of association between catalase gene polymorphism (T/C exon 9) and susceptibility to vitiligo in a Turkish population

H. Bulut, Pehlivan, M., Alper, S., Tomatir, A. G., Onay, H., Yüksel, S. E., Özkinay, F., and Pehlivan, S., Lack of association between catalase gene polymorphism (T/C exon 9) and susceptibility to vitiligo in a Turkish population, vol. 10, pp. 4126-4132, 2011.

Accumulation of hydrogen peroxide (H2O2) and low catalase (CAT) activity have been demonstrated in the epidermis of vitiligo patients. We investigated a possible association between the CAT exon 9 (Asp-389) gene and vitiligo susceptibility in the Turkish population. Thirty-four patients with vitiligo and 49 gender, age and ethnic matched controls were enrolled in the study. Genotyping was done by PCR-RFLP. The CAT exon 9 (Asp-389) genotype and allele frequencies of vitiligo patients did not differ significantly from those of healthy controls.

Assessing genetic variability in bat species of Emballonuridae, Phyllostomidae, Vespertilionidae and Molossidae families (Chiroptera) by RFLP-PCR

S. R. C. Marchesin, Beguelini, M. R., Faria, K. C., Moreira, P. R. L., and Morielle-Versute, E., Assessing genetic variability in bat species of Emballonuridae, Phyllostomidae, Vespertilionidae and Molossidae families (Chiroptera) by RFLP-PCR, vol. 7, pp. 1164-1178, 2008.

A PCR-RFLP analysis of the restriction pattern in nuclear (RAG2) and mitochondrial (12S/16S) gene sequences of bat species from the Molossidae, Phyllostomidae, Vespertilionidae, and Emballonuridae families produced a large number of fragments: 107 for RAG2 and 155 for 12S/16S combined in 139 and 402 haplotypes, respectively. The values detected for gene variation were low for both sequences (0.13 for RAG2 and 0.15 for 12S/16S) and reflected their conservative feature, reinforced by high values of inter- and intraspecies genetic identity (70-100%).

PIT1 gene polymorphism in Pietrain and Large White pigs after divergent selection

A. C. P. Silveira, Braga, T. F., Almeida, J. F., Antunes, R. C., Freitas, P. F. A., César, A. S. M., César, A. S. M., and Guimarães, E. C., PIT1 gene polymorphism in Pietrain and Large White pigs after divergent selection, vol. 8, pp. 1008-1012, 2009.

We examined the polymorphisms in the PIT1 gene of 103 pigs and compared their frequencies in the maternal and paternal lineages of the Pietrain and Large White breeds, which have undergone divergent selection for over 30 years. DNA samples extracted from the blood of these animals were amplified by PCR and genotyped by RFLP, using the restriction enzyme RsaI. The data were analyzed with the chi-square test.

DGAT1 K232A polymorphism in Brazilian cattle breeds

G. A. Lacorte, Machado, M. A., Martinez, M. L., Campos, A. L., Maciel, R. P., Verneque, R. S., Teodoro, R. L., Peixoto, M. G. C. D., Carvalho, M. R. S., and Fonseca, C. G., DGAT1 K232A polymorphism in Brazilian cattle breeds, vol. 5, pp. 475-482, 2006.

Recent reports identified DGAT1 (EC 2.3.1.20) harboring a lysine to alanine substitution (K232A) as a candidate gene with a strong effect on milk production traits. Our objective was to estimate the frequency of the DGAT1 K232A polymorphism in the main Zebu and Taurine breeds in Brazil as well as in Zebu x Taurine crossbreds as a potential QTL for marker-assisted selection.

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