Pepper

Novel genetic male sterility developed in (Capsicum annuum x C. chinense) x C. pubescens and induced by HNO2 showing Mendelian inheritance and aborted at telophase of microspore mother cell stage

W. Huang, Ji, J. - J., Li, C., Li, G. - Q., Yin, C. - C., Chai, W. - G., and Gong, Z. - H., Novel genetic male sterility developed in (Capsicum annuum x C. chinense) x C. pubescens and induced by HNO2 showing Mendelian inheritance and aborted at telophase of microspore mother cell stage, vol. 14, pp. 3318-3329, 2015.

A novel genetic male sterile germplasm was developed by successively crossing of (C. annuum x C. chinense) x C. pubescens and by chemical mutagenesis in pepper. The sterile anthers showed morphological abnormalities, but pistils developed normally with fine pollination capability. We investigated fertility segregation through sib-crossing of the same strains and test crossing by male sterile plants with 6 advanced inbred lines.

Discovery of a large set of SNP and SSR genetic markers by high-throughput sequencing of pepper (Capsicum annuum)

M. Nicolaï, Pisani, C., Bouchet, J. - P., Vuylsteke, M., and Palloix, A., Discovery of a large set of SNP and SSR genetic markers by high-throughput sequencing of pepper (Capsicum annuum), vol. 11. pp. 2295-2300, 2012.

Genetic markers based on single nucleotide polymorphisms (SNPs) are in increasing demand for genome mapping and fingerprinting of breeding populations in crop plants. Recent advances in high-throughput sequencing provide the opportunity for whole-genome resequencing and identification of allelic variants by mapping the reads to a reference genome. However, for many species, such as pepper (Capsicum annuum), a reference genome sequence is not yet available. To this end, we sequenced the C. annuum cv.

Optimization of virus-induced gene silencing in pepper (Capsicum annuum L.)

J. - E. Wang, Li, D. - W., Gong, Z. - H., and Zhang, Y. - L., Optimization of virus-induced gene silencing in pepper (Capsicum annuum L.), vol. 12, pp. 2492-2506, 2013.

Virus-induced gene silencing is currently a powerful tool for the study of gene function in plants. Here, we optimized the protocol for virus-induced gene silencing, and investigated factors that affect the efficiency of tobacco rattle virus-induced gene silencing in pepper plants. Consequently, an optimal protocol was obtained by the syringe-infiltration method in the leaves of pepper plants. The protocol involves 2-leaf stage plants, preparing the Agrobacterium inoculum at a final OD600 of 1.0 and then growing the inoculated plants at 22°C.

Maintaining and restoring cytoplasmic male sterility systems in pepper (Capsicum annuum L.)

Y. Ma, Huang, W., Ji, J. - J., Gong, Z. - H., Yin, C. - C., Ahmed, S. S., and Zhao, Z. - L., Maintaining and restoring cytoplasmic male sterility systems in pepper (Capsicum annuum L.), vol. 12, pp. 2320-2331, 2013.

We studied the efficiency of maintaining and restoring cytoplasmic male sterility (CMS) systems in pepper (Capsicum annuum L.). An Rf-linked molecular marker was employed to analyze the interaction between 6 CMS lines (A), 5 maintainers (B), and 6 restorers (C). Sterility was maintained in the matings of lines 201A x 200B, 203A x 200B, 206A x 200B, 200A x 201B, 206A x 201B, 200A x 202B, 200A x 203B, 200A x 206B, and 201A x 206B.

Physical location of the carotenoid biosynthesis genes Psy and β-Lcy in Capsicum annuum (Solanaceae) using heterologous probes from Citrus sinensis (Rutaceae)

V. Andrade-Souza, Costa, M. G. C., Chen, C. X., Gmitter, Jr., F. G., and Costa, M. A., Physical location of the carotenoid biosynthesis genes Psy and β-Lcy in Capsicum annuum (Solanaceae) using heterologous probes from Citrus sinensis (Rutaceae), vol. 10, pp. 404-409, 2011.

Carotenoids are responsible for a range of fruit colors in different hot pepper (Capsicum) varieties, from white to deep red. Color traits are genetically determined by three loci, Y, C1, and C2, which are associated with carotenogenic genes. Although such genes have been localized on genetic maps of Capsicum and anchored in Lycopersicon and Solanum, physical mapping in Capsicum has been restricted to only a few clusters of some multiple copy genes.

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