pGN plasmid

Construction and functional study of pGN, a mammary gland-specific expression plasmid

J. Lin, Yu, Q. H., Zhang, Q., and Yang, Q., Construction and functional study of pGN, a mammary gland-specific expression plasmid, vol. 13, pp. 4057-4070, 2014.

The aim of this study was to construct a mammary gland-specific expression vector, pGN, and to validate its function in expressing growth hormone (GH) both in vitro and in vivo. First, the GH gene was amplified and inserted downstream of the b-casein 5'-arm. Next, the neo gene was cloned downstream of the b-casein 3'-arm as a selection marker. To analyze the bioactivity of the pGN plasmid, we expressed pGN in a Bcap-37 cell line and in the goat mammary gland.

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