Pig

Molecular characterization, tissue expression profile, and single nucleotide polymorphism analysis of the periostin gene in swine

D. Xu, Ma, H. M., Xiao, D. F., Xu, D., Ma, H. M., and Xiao, D. F., “Molecular characterization, tissue expression profile, and single nucleotide polymorphism analysis of the periostin gene in swine”, vol. 15, p. -, 2016.

Periostin, also called osteoblast-specific factor 2, is an important regulator of bone, cardiac development, and wound healing. A recent study revealed that periostin plays an important role in tumor development and is upregulated in a wide variety of cancers. However, little is known about periostin in swine. Therefore, the cDNA sequence of the porcine periostin gene was obtained by rapid amplification of cDNA ends (RACE).

Differential expression of FUT1 and FUT2 in Large White, Meishan, and Sutai porcine breeds

R. W. Xia, Qin, W. Y., Gan, L. N., Wang, J., Wu, S. L., Bao, W. B., Xia, R. W., Qin, W. Y., Gan, L. N., Wang, J., Wu, S. L., and Bao, W. B., “Differential expression of FUT1 and FUT2 in Large White, Meishan, and Sutai porcine breeds”, vol. 15, p. -, 2016.

To assess the relationship between the expression of a(1,2)-fucosyltransferase (FUT1 and FUT2) genes and resistance to Escherichia coli F18 in weaned pigs, FUT1 and FUT2 expression levels in Large White, Meishan, and Sutai pigs (with resistance to E. coli F18) were determined using real-time PCR. The results revealed that FUT1 and FUT2 expression levels were higher in the liver, lungs, kidneys, stomach, duodenum, and jejunum than in the muscle and heart.

Molecular characterization and tissue expression profile of porcine Ephrin-B2

D. Xu, Ma, H. M., Xu, D., and Ma, H. M., “Molecular characterization and tissue expression profile of porcine Ephrin-B2”, vol. 15, p. -, 2016.

Ephrin-B2 (EFNB2) is a signaling molecule that plays an important role in cell adhesion, proliferation, and migration in humans. However, little is known about this molecule in pigs. In order to investigate whether EFNB2 is associated with the skeletal muscle in pigs, we cloned the full-length cDNA of EFNB2 (GenBank accession No. KF500033) from the longissimus dorsi muscle of Yorkshire pigs by rapid amplification of cDNA ends.

Molecular characterization, chromosome mapping, and expression profile of porcine CDC16

D. Xu, Jiang, J., and Ma, H., “Molecular characterization, chromosome mapping, and expression profile of porcine CDC16”, vol. 14, pp. 12811-12819, 2015.

Cell division cycle16 (CDC16) is a core component among the eight protein subunits of the anaphase-promoting complex (APC). APC is a cyclin degradation system that governs the exit of cells from mitosis. Not much information is available for CDC16 in pig. In this study, a 2284-bp cDNA of porcine CDC16 was obtained by rapid amplification of cDNA ends (RACE). Porcine CDC16 was assigned to SSC11 q11-17, and was determined to be significantly linked with SW1452 by using somatic cell hybrid panel and radiation hybrid panel.

Molecular characterization, tissue expression profile and SNP analysis of porcine GLP2R

Y. L. Chai, Ma, H. M., and Jiang, J., “Molecular characterization, tissue expression profile and SNP analysis of porcine GLP2R”, vol. 14, pp. 12931-12941, 2015.

Glucagon-like peptide-2 receptor (GLP2R), a member of the G-protein-coupled receptor family, plays an important role in intramuscular fat formation. Little is known, however, about porcine GLP2R. In the present study, GLP2R was cloned, and its expression in pig muscle characterized. By rapid amplification of cDNA ends, gene sequence was obtained from Shaziling pigs. Full-length cDNA was 1868 bp, including an open reading frame 1665 bp in length, encoding 554 amino acids, and 203 bp at the 3' end.

Development of FQ-PCR method to determine the level of ADD1 expression in fatty and lean pigs

J. X. Cui, Chen, W., and Zeng, Y. Q., “Development of FQ-PCR method to determine the level of ADD1 expression in fatty and lean pigs”, vol. 14, pp. 13924-13931, 2015.

To determine how adipocyte determination and differentiation factor 1 (ADD1), a gene involved in the determination of pork quality, is regulated in Laiwu and Large pigs, we used TaqMan fluorescence quantitative real-time polymerase chain reaction (FQ-PCR) to detect differential expression in the longissimus muscle of Laiwu (fatty) and Large White (lean) pigs. In this study, the ADD1 and GAPDH cDNA sequences were cloned using a T-A cloning assay, and the clone sequences were consistent with those deposited in GenBank.

Association between ADSL, GARS-AIRS-GART, DGAT1, and DECR1 expression levels and pork meat quality traits

X. D. Zhang, Zhang, S. J., Ding, Y. Y., Feng, Y. F., Zhu, H. Y., Huang, L., Wu, T., Zhou, J., and Yin, Z. J., “Association between ADSL, GARS-AIRS-GART, DGAT1, and DECR1 expression levels and pork meat quality traits”, vol. 14, pp. 14823-14830, 2015.

In this study, meat quality traits were compared between Chinese lard- and European lean-type pigs. The association between expression of four genes (ADSL, GARS-AIRS-GART, DGAT1, and DECR1) and meat quality traits was also investigated. Meat quality traits were found to differ significantly between pig breeds. Meat color parameter values (a* and b*) and intramuscular fat content in Anqingliubai were significantly higher than those in Landrace (P < 0.01).