Plasmodium falciparum

Prevalence of Plasmodium falciparum and P. vivax in an area of transmission located in Pará State, Brazil, determined by amplification of mtDNA using a real-time PCR assay

C. R. T. Souza, Carvalho, T. A. A., Amaral, R. C. G., Cunha, L. S., Cunha, M. G., and Guerreiro, J. F., Prevalence of Plasmodium falciparum and P. vivax in an area of transmission located in Pará State, Brazil, determined by amplification of mtDNA using a real-time PCR assay, vol. 11, pp. 3409-3413, 2012.

The need for a more sensitive and time-efficient assay for malaria has led to the development of molecular assays involving real-time PCR (qPCR), a procedure that has the potential to detect low levels of parasitemia, identify mixed infections, and allow for precise differentiation of species via melting curve analysis or TaqMan fluorescence-labeled probes. Since the first study published in 2001 at least 17 assays have been developed, most of them using SSUrRNA as the target gene. We used qPCR to detect Plasmodium falciparum and P.

Frequency of ABO blood group system polymorphisms in Plasmodium falciparum malaria patients and blood donors from the Brazilian Amazon region

D. B. Carvalho, de Mattos, L. C., Souza-Neiras, W. C., Bonini-Domingos, C. R., Cósimo, A. B., Storti-Melo, L. M., Cassiano, G. C., Couto, A. A. A., Cordeiro, A. J., Rossit, A. R. B., and Machado, R. L. D., Frequency of ABO blood group system polymorphisms in Plasmodium falciparum malaria patients and blood donors from the Brazilian Amazon region, vol. 9, pp. 1443-1449, 2010.

We investigated the ABO genotypes and heterogeneity of the O alleles in Plasmodium falciparum-infected and non-infected individuals from the Brazilian Amazon region. Sample collection took place from May 2003 to August 2005, from P. falciparum malaria patients from four endemic regions of the Brazilian Amazon. The control group consisted of donors from four blood banks in the same areas. DNA was extracted using the Easy-DNATM extraction kit. ABO genotyping was performed using PCR/RFLP. There was a high frequency of ABO*O01O01.

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