Polyclonal antibody

Preparation of polyclonal antibody against porcine beta defensin 2 and identification of its distribution in tissues of pig

Y. Y. Bao, Li, L., Zhang, H., Gao, C. Y., Xiao, C. B., and Li, C. L., Preparation of polyclonal antibody against porcine beta defensin 2 and identification of its distribution in tissues of pig, vol. 14, pp. 18863-18871, 2015.

Porcine β-defensin 2 (pBD2) is an antimicrobial peptide in pigs that plays an important role in the immune system by preventing bacterial invasion. To produce an anti-pBD2 antibody, which is not commercially available, we expressed and purified a soluble, his-tagged version of pBD2 (his-pBD2). Purified pBD2 was injected into New Zealand white rabbits to generate polyclonal antiserum. Anti-pBD2 antibodies were purified by ammonium sulfate precipitation, followed by diethylaminoethyl cellulose ion-exchange chromatography.

Expression and antibody generation of the cancer-testis antigen, BIOT2-S

J. - Y. Wang, Cao, M., Guo, M. - R., Li, S., Yang, X. - F., Wang., M., Fang, J., and Zhao, J., Expression and antibody generation of the cancer-testis antigen, BIOT2-S, vol. 14, pp. 8685-8693, 2015.

Biot2-S is a mouse cancer-testis antigen gene that was identified using the cross-reactive serological analysis of recombinant cDNA expression libraries (SEREX) technique in the State Key Laboratory of Biotherapy, West China Hospital, Sichuan University. To express BIOT2-S and generate its antibody for further investigation, the Biot2-S prokaryotic recombinant expression vector Biot2-S/pGEX6P-1 was constructed with Escherichia coli DH5α as a cloning vector, and BIOT2-S was expressed in E. coli Rosetta (DE3).

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