Polymorphism

Involvement of CYP1A1, GST, 72TP53 polymorphisms in the pathogenesis of thyroid nodules

A. A. S. Reis, Silva, D. M., Curado, M. P., and da Cruz, A. D., Involvement of CYP1A1, GST, 72TP53 polymorphisms in the pathogenesis of thyroid nodules, vol. 9, pp. 2222-2229, 2010.

Specific genotypes appear to be related to the development of thyroid disease. We examined whether polymorphisms of the genes CYP1A1, GSTM1, GSTT1, and TP53 at codon 72 are associated with increased risk for thyroid nodules. Blood samples were obtained from 122 thyroid patients with nodules and from 134 healthy control individuals from Goiânia city, GO, Brazil. We found no significant association of CYP1A1m1 and CYP1A1m2 genotypes with thyroid diseases (P > 0.05).

Genetic polymorphism, molecular characterization and relatedness of Macrobrachium species (Palaemonidae) based on RAPD-PCR

A. L. Guerra, Lima, A. V. B., Taddei, F. G., and Castiglioni, L., Genetic polymorphism, molecular characterization and relatedness of Macrobrachium species (Palaemonidae) based on RAPD-PCR, vol. 9, pp. 2317-2327, 2010.

The prawn genus Macrobrachium belongs to the family Palaemonidae. Its species are widely distributed in lakes, reservoirs, floodplains, and rivers in tropical and subtropical regions of South America. Globally, the genus Macrobrachium includes nearly 210 known species, many of which have economic and ecological importance. We analyzed three species of this genus (M. jelskii, M. amazonicum and M.

Isolation and characterization of eight polymorphic microsatellite loci for the coconut pest, Brontispa longissima (Coleoptera: Hispidae)

C. L. Ma, Wu, H. L., Hu, H. Y., Wu, X., Ma, G. C., Fu, Y. G., and Peng, Z. Q., Isolation and characterization of eight polymorphic microsatellite loci for the coconut pest, Brontispa longissima (Coleoptera: Hispidae), vol. 10. pp. 429-432, 2011.

Brontispa longissima is one of the most serious insect pests of coconut in Southeast Asia; it was first discovered on Hainan Island in June 2002. Despite the economic risk associated with this pest, genetic aspects of the invasion process have remained relatively unexplored. Using microsatellite markers, we investigated the population structure, genetic variability and pattern of invasion in various geographic populations. The methodology was based on a modified biotin-capture method. Eight polymorphic microsatellite loci were isolated and characterized for the pest.

Isolation and characterization of 12 polymorphic microsatellite markers for the frog Pelophylax hubeiensis

J. - C. Yan, Zhu, F. - H., and Wu, H. - L., Isolation and characterization of 12 polymorphic microsatellite markers for the frog Pelophylax hubeiensis, vol. 10. pp. 268-272, 2011.

Twelve polymorphic microsatellite loci were isolated from an (AC)n- and (AG)n-enriched DNA library for the endemic Chinese frog Pelophylax hubeiensis (Ranidae). The number of alleles per locus ranged from two to eight, with a mean of 5.17. The observed and expected heterozygosities ranged from 0.226 to 0.839 and from 0.204 to 0.826, with means of 0.568 and 0.656, respectively. No significant linkage disequilibrium was detected among these loci.

Analysis of the polymorphisms XRCC1Arg194Trp and XRCC1Arg399Gln in gliomas

A. C. Custódio, Almeida, L. O., Pinto, G. R., Santos, M. J., Almeida, J. R. W., Clara, C. A., Rey, J. A., and Casartelli, C., Analysis of the polymorphisms XRCC1Arg194Trp and XRCC1Arg399Gln in gliomas, vol. 10, pp. 1120-1129, 2011.

XRCC genes (X-ray cross-complementing group) were discovered mainly for their roles in protecting mammalian cells against damage caused by ionizing radiation. Studies determined that these genes are important in the genetic stability of DNA. Although the loss of some of these genes does not necessarily confer high levels of sensitivity to radiation, they have been found to represent important components of various pathways of DNA repair. To ensure the integrity of the genome, a complex system of DNA repair was developed.

Ala-9Val polymorphism of Mn-SOD gene in sickle cell anemia

S. Sogut, Yonden, Z., Kaya, H., Oktar, S., Tutanc, M., Yilmaz, H. R., Yigit, A., Ozcelik, N., and Gali, E., Ala-9Val polymorphism of Mn-SOD gene in sickle cell anemia, vol. 10, pp. 828-833, 2011.

Oxidative stress may be contributory to the pathophy­siology of the abnormalities that underlie the clinical course of sickle cell anemia. We looked for a possible genetic association between the functional polymorphism Ala-9Val in the human Mn-SOD gene and sickle cell anemia. One hundred and twenty-seven patients with sickle cell anemia and 127 healthy controls were recruited into the study.

Cox-2 gene polymorphism and IL-6 levels in coronary artery disease

K. K. Ol, Agachan, B., Gormus, U., Toptas, B., and Isbir, T., Cox-2 gene polymorphism and IL-6 levels in coronary artery disease, vol. 10, pp. 810-816, 2011.

Coronary artery disease is one of the leading causes of mortality and diabetes mellitus is one of its main risk factors due to microvascular and macrovascular complications, such as atherosclerosis. Atherosclerosis is now known to be an inflammatory process mediated by prostaglandins and several interleukins. As both are important in inflammatory processes, we examined Cox-2 (-765G > C) polymorphism and interleukin-6 levels in coronary artery disease patients compared to healthy controls.

Detection of differentially expressed genes in the longissimus dorsi of Northeastern Indigenous and Large White pigs

Y. Gao, Zhang, Y. H., Jiang, H., Xiao, S. Q., Wang, S., Ma, Q., Sun, G. J., Li, F. J., Deng, Q., Dai, L. S., Zhao, Z. H., Cui, X. S., Zhang, S. M., Liu, D. F., and Zhang, J. B., Detection of differentially expressed genes in the longissimus dorsi of Northeastern Indigenous and Large White pigs, vol. 10, pp. 779-791, 2011.

Recent attention in pig breeding programs has focused on the improvement of pork quality in response to increasing consumer demands. Compared to the fatty-type Northeastern Indigenous (Chinese) breed of pigs, the lean-type Large White has lower intramuscular fat and inferior eating quality from the perspective of the Chinese consumer. In order to investigate the molecular basis of differences in pork quality in Chinese indigenous and Western breeds, longissimus dorsi samples were collected from three adult Northeastern Indigenous and three adult Large White pigs.

Polymorphisms of three new microsatellite sites of the dystrophin gene

R. F. Sun, Zhu, Y. S., Feng, J. L., Tian, Z., Kuang, W. J., Liu, Y., Zhang, H. B., and Li, S. B., Polymorphisms of three new microsatellite sites of the dystrophin gene, vol. 10, pp. 744-751, 2011.

To look for novel microsatellites in the dystrophin gene for the diagnosis of Duchenne muscular dystrophy, candidate microsatellite sites in the dystrophin gene were analyzed with the SSRHunter software and were also genotyped. Among the 15 candidate microsatellite sites, three novel microsatellite sites in the 60th, 30th, and 2nd intron were found to have a high degree of polymorphism. We submitted these three new loci to the European Molecular Biology Laboratory, under accession Nos.

Development and testing of 13 polymorphic microsatellite markers in Larimichthys polyactis (Sciaenidae) using 5' anchored PCR

C. Y. Ma, Ma, H. Y., and Ma, L. B., Development and testing of 13 polymorphic microsatellite markers in Larimichthys polyactis (Sciaenidae) using 5' anchored PCR, vol. 10. pp. 1455-1460, 2011.

Larimichthys polyactis is a commercially important marine fish species in southeast Asia. The population crashed due to overfishing in the 1970s, but has since recovered. We developed 13 novel polymorphic microsatellite markers in L. polyactis using 5' anchored PCR. The characteristics of these loci were estimated by analyzing a sample of 30 individuals. A total of 74 alleles were detected, with a mean of 5.7 alleles per locus.

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