Polyploidy

Segregation analysis of microsatellite (SSR) markers in sugarcane polyploids

X. Lu, Zhou, H., Pan, Y. - B., Chen, C. Y., Zhu, J. R., Chen, P. H., Li, Y. - R., Cai, Q., and Chen, R. K., Segregation analysis of microsatellite (SSR) markers in sugarcane polyploids, vol. 14, pp. 18384-18395, 2015.

No information is available on segregation analysis of DNA markers involving both pollen and self-progeny. Therefore, we used capillary electrophoresis- and fluorescence-based DNA fingerprinting together with single pollen collection and polymerase chain reaction (PCR) to investigate simple sequence repeat (SSR) marker segregation among 964 single pollens and 288 self-progenies (S1) of sugarcane cultivar LCP 85-384. Twenty SSR DNA fragments (alleles) were amplified by five polymorphic SSR markers. Only one non-parental SSR allele was observed in 2392 PCRs.

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