Polysaccharides

Isolation, identification, and antioxidant activity of polysaccharides from the shell of abalone (Haliotis discus hannai Ino)

Z. L. Wang, Liang, H. B., Guo, W., Peng, Z. F., Chen, J. D., and Zhang, Q. Q., Isolation, identification, and antioxidant activity of polysaccharides from the shell of abalone (Haliotis discus hannai Ino), vol. 13, pp. 4883-4892, 2014.

In this study, two antioxidative substances, a homogeneous polysaccharide [abalone shell polysaccharide (ASP-1), corresponding to the first peak by size exclusion chromatography] and a non-polysaccharide compound [abalone shell compound (ACS-2), corresponding to the second peak by size exclusion chromatography], were extracted from the abalone (Haliotis discus hannai Ino) shell. We primarily focused on the investigation of ASP-1.

A generic plant RNA isolation method suitable for RNA-Seq and suppression subtractive hybridization

Y. Q. Zhu, Wu, W. J., Xiao, H. W., Chen, H. B., Zheng, Y., Zhang, Y. J., .X.Wang, H., and Huang, L. Q., A generic plant RNA isolation method suitable for RNA-Seq and suppression subtractive hybridization, vol. 12, pp. 5537-5546, 2013.

A recently developed revolutionary approach to transcrip­tomics, RNA-Seq, and suppression subtractive hybridization are power­ful tools for gene expression research. However, currently, the difficulty of isolating high-quality RNAs from plant tissues bearing abundant complex polysaccharides, polyphenolics, and secondary metabolites is a serious problem that not only limits the application of these technologies but also hinders studies dealing with RNA in general.

Isolation of retro-transcribed RNA from in vitro Mycosphaerella fijiensis-infected banana leaves

C. M. Rodríguez-García, Peraza-Echeverría, L., Islas-Flores, I. R., Canto-Canché, B. B., and Grijalva-Arango, R., Isolation of retro-transcribed RNA from in vitro Mycosphaerella fijiensis-infected banana leaves, vol. 9, pp. 1460-1468, 2010.

High polyphenol and polysaccharide levels in plant tissues such as banana fruit and leaves constitute a significant challenge to the extraction of sufficient amounts of high-quality RNA required for cDNA library synthesis and molecular analysis.

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