Population structure

Genetic bottlenecks in Turkish okra germplasm and utility of iPBS retrotransposon markers for genetic diversity assessment

M. Yildiz, Koçak, M., and Baloch, F. S., “Genetic bottlenecks in Turkish okra germplasm and utility of iPBS retrotransposon markers for genetic diversity assessment”, vol. 14, pp. 10588-10602, 2015.

Lack of requisite genetic variation in Turkish okra has necessitated the use of different types of markers for estimating the genetic diversity and identifying the source of variation. Transposable elements, present abundantly in plant genomes, generate genomic diversity through their replication and are thus an excellent source of molecular markers. We hypothesized that inter-primer binding site (iPBS)-retrotransposons could be the source of variation because of their genome plasticity nature.

Isolation and characterization of novel polymorphic microsatellite markers for Lutjanus erythropterus

L. Lin, Li, C. H., Xu, S. N., Liu, Y., and Xiao, Y. Y., “Isolation and characterization of novel polymorphic microsatellite markers for Lutjanus erythropterus”, vol. 14, pp. 10944-10947, 2015.

We isolated and characterized 22 polymorphic microsatellite loci in the crimson snapper (Lutjanus erythropterus) using a (GT)13-enriched genomic library. We found three to 15 alleles per locus, with a mean of 6.68. The observed and expected heterozygosities ranged from 0.087 to 0.978 and from 0.125 to 0.904, respectively, with averages of 0.576 and 0.650, respectively. Only three loci showed significant deviation from the Hardy-Weinberg equilibrium after Bonferroni correction. Four loci showed evidence for null alleles.

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