Potato

Isolation and characterization of a chalcone isomerase gene promoter from potato cultivars

M. Chen, Zhu, W. J., You, X., Liu, Y. D., Kaleri, G. M., and Yang, Q., Isolation and characterization of a chalcone isomerase gene promoter from potato cultivars, vol. 14, pp. 18872-18885, 2015.

Chalcone isomerase (CHI) is a key enzyme involved in anthocyanin metabolism. Previous research on CHI has mainly focused on cDNA cloning and gene expression. In the current study, the 1425-bp potato CHI promoter (PCP) was isolated from four potato cultivars (Heijingang, Zhongshu 7, Désirée, and Favorita) using PCR and DNA sequencing. The PCP contained many cis-regulatory elements (CREs) related to anthocyanin metabolism, tissue specificity, light response, stress, and hormone induction.

A phytotoxin Solanapyrone-A downregulates calcium-dependent protein kinase activity in potato

A. Hassan, Hatsugai, N., and Shah, M. M., A phytotoxin Solanapyrone-A downregulates calcium-dependent protein kinase activity in potato, vol. 12, pp. 1540-1545, 2013.

We previously demonstrated that alternaric acid, a host-specific toxin produced by the plant pathogenic fungus Alternaria solani, in the presence of Ca2+ and Mg2+, stimulated in vitro phosphorylation of His-tagged calcium-dependent protein kinase 2 from potato cultivar Rishiri (RiCDPK2). Herein, we report that Solanapyrone-A (SpA), a non-host-specific toxin produced by A. solani, inhibited the phosphorylation of RiCDPK2 in the presence of Ca2+ and Mg2+.

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