Primary hepatocyte

Attenuated mRNA expression of lipid metabolism genes in primary hepatocytes following lipopolysaccharide treatment in dairy cows

Y. Y. Wang, Li, H. P., Wang, X. J., Jiang, Q. D., Geng, J., Wang, L. F., Guo, Y. J., Lu, W. F., Zhu, H. S., and Yang, G. Y., “Attenuated mRNA expression of lipid metabolism genes in primary hepatocytes following lipopolysaccharide treatment in dairy cows”, vol. 14, pp. 3718-3728, 2015.

The influence of ruminal acidosis on ruminal microbiology and metabolite production has received considerable attention, but little is known regarding the systemic manifestations that arise from ruminal acidosis. Lipopolysaccharide (LPS) is released in the gastrointestinal tract upon ingestion of high-grain or high-fat diets, and it has been implicated in the etiology of multiple energy- and lipid-related metabolic disturbances in ruminants. The liver plays a crucial role in the acute phase response to intruding pathogens.

Isolation and identification of bovine primary hepatocytes

Q. D. Jiang, Li, H. P., Liu, F. J., Wang, X. J., Guo, Y. J., Wang, L. F., Lu, W. F., Li, H. J., Li, X. P., and Yang, G. Y., “Isolation and identification of bovine primary hepatocytes”, vol. 12, pp. 5186-5194, 2013.

The liver is a unique organ that is endowed with a plethora of specialized functions. Most of its functional traits are controlled by hepatocytes. Primary hepatocytes have been used widely in in vitro models to understand the biological processes occurring in the liver. There are a number of methods used to separate hepatocytes, but the cell activity and purity are much lower in this condition. On the basis of previous research, in this study, the two-step collagenase perfusion technique was used for isolating hepatocytes.

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