Proacrosin

Expression and tyrosine phosphorylation of sp32 regulate the activation of the boar proacrosin/acrosin system

H. T. Dong, Shi, W. S., Tian, Y., Cao, L. P., and Jin, Y., “Expression and tyrosine phosphorylation of sp32 regulate the activation of the boar proacrosin/acrosin system”, vol. 14, pp. 2374-2383, 2015.

The current study investigated the relationship between the level of expression and tyrosine phosphorylation of the sperm protein 32 (sp32) and the activation of the boar proacrosin/acrosin system. The acrosomal membrane proteins of boar sperm for use in different treatment experiments (i.e., fresh sperm, freezing and thawing, capacitation, and acrosome reaction) were separated, stained by Coomassie brilliant blue, and analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot.

Proacrosin activation mechanisms in capacitated and frozen-thawed boar spermatozoa

J. J. Cui, Tian, Y., Liu, Y., Huang, H. L., and Jin, Y., “Proacrosin activation mechanisms in capacitated and frozen-thawed boar spermatozoa”, vol. 13, pp. 9915-9920, 2014.

The main objective of the current study was to explore the different activation mechanisms of capacitation and freeze-thawed spermatozoa. Using SDS-PAGE and Western blotting, the conversion process of boar proacrosin during freeze-thawing and capacitation of spermatozoa was analyzed. The results revealed that capacitated spermatozoa exhibited a greater fluorescence area than that of the freeze-thawed spermatozoa, which were smaller than those of the fresh group.

Activation of proacrosin accompanies upregulation of sp32 protein tyrosine phosphorylation in pig sperm

P. L. Sun, Yang, L. X., Cui, J. - J., Tian, Y., Liu, Y., and Jin, Y., “Activation of proacrosin accompanies upregulation of sp32 protein tyrosine phosphorylation in pig sperm”, vol. 12, pp. 6579-6587, 2013.

This study investigated the relationship between acrosin activation and pig sperm proacrosin binding protein (sp32) phosphorylation levels. Differently processed pig spermatozoa (fresh semen sperm, capacitation sperm, acrosome reaction sperm, capacitation-like sperm, and thawed sperm) were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot analysis.

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