Promoter DNA methylation

Aberrant promoter methylation of the CD4 gene in peripheral blood cells of mastitic dairy cows

X. S. Wang, Zhang, Y., He, Y. H., Ma, P. P., Fan, L. J., Wang, Y. C., Zhang, Y. I., Sun, D. X., Zhang, S. L., Wang, C. D., Song, J. Z., and Yu, Y., Aberrant promoter methylation of the CD4 gene in peripheral blood cells of mastitic dairy cows, vol. 12, pp. 6228-6239, 2013.

Bovine mastitis is the most common and costly disease of dairy cattle. Cluster of differentiation 4 (CD4) is closely related to the immune response in mastitis. We quantified promoter CpG methylation levels of the CD4 gene in Chinese Holsteins with clinical mastitis (CM) and in healthy controls; these levels were quantitatively detected with bisulfite pyrosequencing assays and confirmed by cloning sequencing. We found that the bovine CD4 promoter had 16% more methyl groups in the cows with CM (75.0 ± 5.8%) compared to the controls (59.0 ± 8.5%).

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