M. Alam, M. Mahajan, M. Raziuddin, T.P. Singh, S. Yadav
Published: August 04, 2009
Genet. Mol. Res. 8 (3) : 929-937
DOI: https://doi.org/10.4238/vol8-3gmr620
Cite this Article:
M. Alam, M. Mahajan, M. Raziuddin, T.P. Singh, S. Yadav (2009). Proteomics-based approach for identification and purification of human phosphate binding apolipoprotein from amniotic fluid. Genet. Mol. Res. 8(3): 929-937. https://doi.org/10.4238/vol8-3gmr620
About the Authors
M. Alam, M. Mahajan, M. Raziuddin, T.P. Singh, S. Yadav
Corresponding Author
S. Yadav
Email: savita11@gmail.com
ABSTRACT
Human amniotic fluid is of both maternal and fetal origin; it protects the fetus and provides the environment for growth and development of the fetus. We used a proteomics-based approach for targeting and purifying human phosphate binding protein, a member of the DING family of proteins from amniotic fluid, using Blue Sepharose CL-6B, DEAE-Sephacel and gel filtration chromatography. The protein had earlier been reported to be serendipitously purified along with PON1 (paraoxonase 1). It was identified using electro-spray-ionization-time-of-flight mass spectrometry and was found to be human phosphate binding protein. Human phosphate binding proteins have been reported to play a role as phosphate scavengers and may have a protective function against phosphate-related disorders, such as atherosclerosis, diabetes and kidney stones.
Key words: Proteomics, Amniotic fluid, Human phosphate binding protein.