Proteomics

Proteomic analysis of susceptibility in intestinal stromal tumors

Y. C. Yang, Cai, X., Zhang, S. Y., Jia, Z. L., Chen, T. B., Liu, L. R., Liu, J., and Zhao, M. C., “Proteomic analysis of susceptibility in intestinal stromal tumors”, vol. 14, pp. 13566-13571, 2015.

We analyzed the susceptibility of intestinal stromal tumors using cell culture and proteomics. Human SGC7901 gastric cells were selected and divided into a blank control group (untransfected SGC7901 cells), a negative control group [SGC7901 cells transfected with negative interference control-small interfering RNA (siRNA)], and a COOH-terminus tensin-like molecule (CTEN)-siRNA-1 group (SGC7901 cells transfected with CTEN-siRNA-1). The cells were successfully transfected and subjected to analyses of cell proliferation, cell cycle, cell invasion, CTEN expression, and proteomics.

Protein extraction method for the proteomic study of Zymomonas mobilis during production of ethanol and levans

D. R. Cavalcanti, Malafaia, C. B., Silva, T. D., Santos, B. S., Calazans, G. M. T., and Silva, M. V., “Protein extraction method for the proteomic study of Zymomonas mobilis during production of ethanol and levans”, vol. 14, pp. 14406-14421, 2015.

Zymomonas mobilis has aroused considerable interest owing to its rapid metabolism and efficiency in producing ethanol and by-products such as levans, sorbitol, and gluconic acid from simple sugars. We performed a proteomic analysis of Z. mobilis UFPEDA241 to provide a global profile of regulatory proteins. The choice of the methods of extraction and cell lysis are fundamental steps and of great importance for the detection and identification of intra- and extracellular proteins of a proteome.