Purification

Optimization of the procedure for extracting nucleic acids from aloe

J. Li, Zhang, Y. S., Liao, C. M., and Liu, X. H., Optimization of the procedure for extracting nucleic acids from aloe, vol. 13, pp. 276-282, 2014.

Aloe, an important folk herbal drug, includes abundant polysaccharides and secondary metabolites, which make it difficult to isolate high-quality DNA or RNA. In this paper, one and two improved methods were used to isolate the genomic DNA and RNA from the leaf of aloe, respectively. The obtained samples presented good quality and integrity; thus, they could be further used for many downstream molecular experiments. These reported protocols for DNA and RNA extraction offered a valuable reference for other related studies.

Separation, purification, and identification of flagellin, and preparation of its antisera

M. D. Hu, Wang, G. S., Xu, J., Yao, W., He, B. F., Yang, Y., Mao, M., Wang, Q., and Xu, J. C., Separation, purification, and identification of flagellin, and preparation of its antisera, vol. 13, pp. 9161-9170, 2014.

The aim of this study was to separate, purify, and identify Salmonella paratyphi A flagellin, and to prepare its antisera. Primary flagellin was isolated from S. paratyphi A using the acid lysis method. The flagellin was purified with weak anion exchange chromatography and the protein was identified with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blot, and negative staining with phosphotungstic acid with scanning electron microscopy (SEM). The production of the obtained flagellin was then quantified.

Overexpression, purification, and pharmacologic evaluation of anticancer activity of ribosomal protein L24 from the giant panda (Ailuropoda melanoleuca)

Y. L. Hou, Ding, X., Hou, W., Song, B., Wang, T., Wang, F., Li, J., Zhong, J., Xu, T., Ma, B. X., Zhu, H. Q., Li, J. H., and Zhong, J. C., Overexpression, purification, and pharmacologic evaluation of anticancer activity of ribosomal protein L24 from the giant panda (Ailuropoda melanoleuca), vol. 12, pp. 4735-4750, 2013.

The ribosomal protein L24 (RPL24) belongs to the L24E family of ribosomal proteins and is located in the cytoplasm. The purpose of this study was to investigate the structure and anti-cancer function of RPL24 of the giant panda (Ailuropoda melanoleuca). The complementary DNA of RPL24 was cloned successfully using reverse transcription-polymerase chain reaction technology. We constructed a recombinant expression vector containing RPL24 complementary DNA and overexpressed it in Escherichia coli using pET28a plasmids.

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