Qinchuan cattle

Efficient construction of recombinant adenovirus expression vector of the Qinchuan cattle LYRM1 gene

Y. K. Li, Fu, C. Z., Zhang, Y. R., and Zan, L. S., Efficient construction of recombinant adenovirus expression vector of the Qinchuan cattle LYRM1 gene, vol. 14, pp. 9469-9477, 2015.

In this study, we cloned the coding DNA sequence (CDS) region of Qinchuan cattle LYR motif-containing 1 (LYRM1) and constructed a recombinant adenovirus expression vector to examine the function of LYRM1 on the cellular level. Total RNA was extracted from the adipose tissue of Qinchuan cattle, cDNA was obtained by reverse transcription, and polymerase chain reaction was used to amplify the CDS region of the LYRM1 gene. The CDS-containing fragment was inserted into the shuttle vector pAdTrack-CMV to construct pAdTrack-CMV-LYRM1 vector.

Novel polymorphisms of the PRKAG2 gene and their association with body measurement and meat quality traits in Qinchuan cattle

C. Z. Fu, Tian, W. Q., Li, Y. K., Wei, S. J., Cheng, G., Wang, H. B., and Zan, L. S., Novel polymorphisms of the PRKAG2 gene and their association with body measurement and meat quality traits in Qinchuan cattle, vol. 14, pp. 3669-3679, 2015.

Body measurement and meat quality traits play important roles in the evaluation of productivity and economy in cattle, which are influenced by genes and environmental factors. PRKAG2, which encodes the γ2 regulatory subunit of AMPK, is associated with key metabolic pathways in muscle. We detected bovine PRKAG2 gene polymorphisms and analyzed their associations with body measurement and meat quality traits of cattle. DNA samples were taken from 578 Qinchuan cattle aged 18-24 months.

Polymorphisms of the Osteocrin gene and its association with meat quality traits in Qinchuan cattle

Y. F. Huangfu, Zan, L. S., Adoligbe, C., Wang, H. B., Wang, H., and Gao, J. B., Polymorphisms of the Osteocrin gene and its association with meat quality traits in Qinchuan cattle, vol. 14, pp. 4890-4895, 2015.

Here, we detected 2 SNPs, A85C and T335C, that were located on the 3rd exon and the 3 untranslated regions of the bovine Osteocrin gene, respectively, using 413 Qinchuan cattle DNA samples. PCR-SSCP and DNA sequencing methods were specifically used. Three genotypes (AA, AC, and CC) were found at A85C; yet, only 2 genotypes (TC and CC) were found at T335C. Association analysis showed that both loci were associated with certain meat quality traits, including back fat thickness and loin muscle area. At the A85C locus, individuals with the CC genotype had greater back fat thickness.

Polymorphisms in the delta-like 2 homolog gene and their association with growth and meat-quality traits in Qinchuan cattle

N. Yang, Zan, L. S., Li, Y. K., Gao, J. B., Ma, X. H., Fu, C. Z., Wang, H., and Adoligbe, C., Polymorphisms in the delta-like 2 homolog gene and their association with growth and meat-quality traits in Qinchuan cattle, vol. 13, pp. 2130-2139, 2014.

The delta-like 2 homolog (DLK2) modulates adipogenesis, hematopoiesis, osteogenesis, and other cell-differentiation processes. In the present study, we detected potential polymorphisms in the DLK2 gene in 604 individuals of Qinchuan cattle by using PCR-RFLP and DNA-sequencing methods. Herein, we identified five novel single-nucleotide polymorphisms (SNPs) (g.888G>A, g.910A>G, g.995G>A, g.4321A>G, g.4850A>G) and analyzed their association with measured traits.

Investigation of the association of two candidate genes (H-FABP and PSMC1) with growth and carcass traits in Qinchuan beef cattle from China

W. Liang, Zhang, H. L., Liu, Y., Lu, B. C., Liu, X., Li, Q., and Cao, Y., Investigation of the association of two candidate genes (H-FABP and PSMC1) with growth and carcass traits in Qinchuan beef cattle from China, vol. 13, pp. 1876-1884, 2014.

Growth and carcass traits are economically important quality characteristics of beef cattle and are complex quantitative traits that are controlled by multiple genes. In this study, 2 candidate genes, H-FABP (encoding the heart fatty acid-binding protein) and PSMC1 (encoding the proteasome 26S subunit of ATPase 1) were investigated in Qinchuan beef cattle of China.

New polymorphisms in the novel LYRM1 gene are associated with body measurement and meat quality traits in Qinchuan cattle

Y. K. Li, Huangpu, Y. F., Gao, J. B., Yang, N., Fu, C. Z., Wang, H. B., Cheng, G., and Zan, L. S., New polymorphisms in the novel LYRM1 gene are associated with body measurement and meat quality traits in Qinchuan cattle, vol. 13, pp. 6926-6936, 2014.

Body measurement and meat quality traits play important roles in the evaluation of productivity in cattle; they are influenced by genetic and environmental factors. Recent studies have shown that LYRM1 is a novel gene related to obesity and may influence fat deposition. We screened for new polymorphisms in the bovine LYRM1 gene and analyzed their association with body measurement and meat quality traits in cattle. DNA samples were obtained from 572 Qinchuan cattle aged from 18 to 24 months. DNA sequencing was used to find the LYRM1 single nucleotide polymorphisms (SNPs).

Effects of bovine SMO gene polymorphisms on the body measurement and meat quality traits of Qinchuan cattle

Y. R. Zhang, Li, Y. K., Fu, C. Z., Wang, J. L., Wang, H. B., and Zan, L. S., Effects of bovine SMO gene polymorphisms on the body measurement and meat quality traits of Qinchuan cattle, vol. 13, pp. 8105-8117, 2014.

Beef cattle breeding programs focus on improving important economic traits, including growth rates, and meat quantity and quality. Molecular marker-assisted selection based on genetic variation represents a potential method for breeding genetically improved livestock with better economic traits. Smoothened (SMO) protein is a signal transducer that contributes to the regulation of both osteogenesis and adipogenesis through the hedgehog pathway.

Four SNPs of insulin-induced gene 1 associated with growth and carcass traits in Qinchuan cattle in China

Y. Liu, Liu, X. L., He, H., and Gu, Y. L., Four SNPs of insulin-induced gene 1 associated with growth and carcass traits in Qinchuan cattle in China, vol. 11, pp. 1209-1216, 2012.

The insulin-induced gene 1 (Insig-1) is a regulator of lipid metabolism and plays an important role in the sterol-mediated regulation of SREBP, SCAP and HMG-CoA reductase. We used PCR-RFLP and DNA sequencing to detect polymorphisms of the Insig-1 gene in 215 individuals of the Qinchuan cattle breed. Four SNPs [4366(A>G), 4534(T>C), 5001(T>C), and 5235(G>A)] were indentified. The association of the genetic viariation with growth and carcass traits (body length, withers height, hip width, slaughter weight, and carcass weight) was analyzed.

Cloning, expression analysis and sequence prediction of the CCAAT/enhancer-binding protein alpha gene of Qinchuan cattle

H. Wang, Zan, L. S., Wang, H. B., Gong, C., and Fu, C. Z., Cloning, expression analysis and sequence prediction of the CCAAT/enhancer-binding protein alpha gene of Qinchuan cattle, vol. 11, pp. 1651-1661, 2012.

CCAAT/enhancer-binding protein alpha (C/EBPα) is an essential transcription factor, regulating the differentiation of adipocytes. We cloned the complete open reading frame of C/EBPα gene of Qinchuan cattle and analyzed its protein structures and expression profile in 15 tissues via DNA cloning, sequencing and RT-PCR. Analysis of the putative protein sequences revealed that C/EBPα consists of alpha helices, random coils and a few extended strands. A significant transmembrane structure was observed in amino acid region 233 to 252.

Comparative analysis of myostatin gene and promoter sequences of Qinchuan and Red Angus cattle

Y. L. He, Wu, Y. H., Quan, F. S., Liu, Y. G., and Zhang, Y., Comparative analysis of myostatin gene and promoter sequences of Qinchuan and Red Angus cattle, vol. 12, pp. 3398-3406, 2013.

To better understand the function of the myostatin gene and its promoter region in bovine, we amplified and sequenced the myostatin gene and promoter from the blood of Qinchuan and Red Angus cattle by using polymerase chain reaction. The sequences of Qinchuan and Red Angus cattle were compared with those of other cattle breeds available in GenBank. Exon splice sites were confirmed by mRNA sequencing. Compared to the published sequence (GenBank accession No.

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