qPCR

A novel rare copy number variant of the ABCF1 gene identified among dengue fever patients from Peninsular Malaysia

B. P. Hoh, Sam, S. S., Umi, S. H., Mahiran, M., Khairudin, N. Y. Nik, S. Hanim, R., and AbuBakar, S., “A novel rare copy number variant of the ABCF1 gene identified among dengue fever patients from Peninsular Malaysia”, vol. 13, pp. 980-985, 2014.

Copy number variation (CNV) is a form of genetic variation in addition to single nucleotide polymorphisms. The significance of CNV in the manifestation of a number of diseases is only recently receiving considerable attention. We genotyped 163 dengue patients from Peninsular Malaysia for genes possibly linked to dengue infection using quantitative real-time PCR. Here, we report a serendipitous discovery of a novel rare CNV of the ABCF1 gene among the dengue patients.

Identification and assessment of differentially expressed genes involved in growth regulation in Apostichopus japonicus

L. Zhu, Li, C. H., Su, X. R., Guo, C. Y., Wang, Z., Jin, C. H., Li, Y., and Li, T. W., “Identification and assessment of differentially expressed genes involved in growth regulation in Apostichopus japonicus”, vol. 12, pp. 3028-3037, 2013.

Rapid and efficient growth is a major consideration and challenge for global mariculture. The differential growth rate of the sea cucumber, Apostichopus japonicus, has significantly hampered the total production of the industry. In the present study, forward and reverse suppression subtractive hybridization libraries were constructed and sequenced from a fast-growth group and a slow-growth group of the sea cucumber. A total of 142 differentially expressed sequence tags (ESTs) with insertions longer than 150 bp were identified and further analyzed.

Analysis of SLC11A1 gene expression in healthy water buffalo (Bubalus bubalis) blood cells using qPCR

A. Crisà, De Matteis, G., Scatà, M. C., and Moioli, B., “Analysis of SLC11A1 gene expression in healthy water buffalo (Bubalus bubalis) blood cells using qPCR”, vol. 12, pp. 6957-6967, 2013.

SLC11A1 (solute carrier family 11 member 1 protein) gene influences the initial phase of bacterial cellular infections through macrophage activation. Recent literature on buffalo has attempted to associate the genotype of the polymorphic microsatellite located in the 3ꞌuntranslated region (3ꞌUTR) of the gene, with either susceptibility to brucellosis or with improved macrophage function. Carriers of the (GT)₁₆ allele have been reported to be resistant to brucellosis.