Quantitative real-time RT-PCR

Characterization of a novel anther-specific gene encoding a leucine-rich repeat protein in petunia

Y. Z. Yue, Sun, J., Huang, X., Peng, H., Liu, G. F., and Hu, H. R., Characterization of a novel anther-specific gene encoding a leucine-rich repeat protein in petunia, vol. 13, pp. 9889-9898, 2014.

In Petunia x hybrida ‘Fantasy Red’, a leucine-rich repeat (LRR) gene referred to as PhLRR, was identified in a flower bud cDNA library. The open reading frame sequence of PhLRR was 1251 bp, encoding a putative 46.2-kDa protein of 416 amino acids. The PhLRR protein showed high similarity to members of polygalacturonase inhibitor proteins (PGIPs), contained 11 conserved LRR domains, and was an extracellular localization protein.

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