Performance of peanut mutants and their offspring generated from mixed high-energy particle field radiation and tissue culture
, “Performance of peanut mutants and their offspring generated from mixed high-energy particle field radiation and tissue culture”, vol. 14, pp. 10837-10848, 2015.
To develop new ways to breed peanut, we irradiated seeds of the Luhua 11 cultivar with a mixed high-energy particle field at different doses. The embryonic leaflets were extracted as explants and incubated on somatic embryo induction medium and then on somatic embryo germination and regeneration medium. After being grafted, the M1-generation plants were transplanted, and seeds from each M1-generation plant were harvested. In the following year, the M2-generation seeds were planted separately.
