RAPD analysis

Vegetative compatibility and genetic analysis of Colletotrichum lindemuthianum isolates from Brazil

Q. L. Barcelos, Souza, E. A., and Silva, K. J. Damasceno, Vegetative compatibility and genetic analysis of Colletotrichum lindemuthianum isolates from Brazil, vol. 10, pp. 230-242, 2011.

The causal agent of common bean anthracnose, Colletotrichum lindemuthianum, has considerable genetic and pathogenic variability, which makes the development of resistant cultivars difficult. We examined variability within and between Brazilian pathotypes of C. lindemuthianum through the identification of vegetative compatibility groups (VCGs) and by RAPD analysis. Two hundred and ninety-five nit mutants were obtained from 47 isolates of various pathotypes of the fungus collected from different regions, host cultivars and years.

Molecular characterization of urdbean (Vigna mungo) germplasm related to resistance against urdbean leaf crinkle virus

R. Binyamin, M. Khan, A., Khan, A. I., M. Khan, A., Awan, F. S., and Khan, N. A., Molecular characterization of urdbean (Vigna mungo) germplasm related to resistance against urdbean leaf crinkle virus, vol. 10, pp. 1681-1688, 2011.

Urdbean (Vigna mungo) is an important pulse crop grown worldwide. Urdbean leaf crinkle virus (ULCV) is a pathogen of urdbean found in Pakistan that causes huge losses in yield. Forty urdbean varieties/lines were screened against the virus under field conditions during spring season 2009. None of the lines appeared to be highly resistant or resistant. On the basis of a 0-5 disease rating scale and disease severity index, genotypes varied significantly in their reaction to ULCV.

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