RAPD

Study of genetic divergence among wheat genotypes through random amplified polymorphic DNA

A. Iqbal, Khan, A. S., Khan, I. A., Awan, F. S., Ahmad, A., and Khan, A. A., Study of genetic divergence among wheat genotypes through random amplified polymorphic DNA, vol. 6, pp. 476-481, 2007.

The degree of genetic divergence was estimated in seven wheat genotypes, six exotic genotypes and one local variety, through random amplified polymorphic DNA methodology. A total of 112 DNA fragments were generated by the 15 random primers, with an average of about 7.4 bands per primer. Among the 112, 50 fragments showed polymorphism among the seven wheat genotypes. Nei and Li’s similarity matrix ranged from 86.2 to 93.0%, which indicated a narrow genetic base among the genotypes. The maximum similarity, 93.0%, was observed between 12WLRG/1-12 and WL-43.

An optimized mini-preparation method to obtain high-quality genomic DNA frommature leaves of sunflower

J. T. Li, Chen, D. C., Zhang, X. L., Tang, Z. S., and Yang, J., An optimized mini-preparation method to obtain high-quality genomic DNA frommature leaves of sunflower, vol. 6, pp. 1064-1071, 2007.

In order to investigate the mutation characteristics and to further examine the genetic variation of mutant sunflower (Helianthus annuus) obtained in plants grown from seeds exposed to space conditions, only the mature tissues such as leaf and flower could be used for DNA extraction after mutation characteristics were confirmed. The rich contents of polysaccharides, tannins, secondary metabolites, and polyphenolics made it difficult to isolate high-quality DNA from mature leaves of sunflower according to previous reports.

Intraspecific variability of Bipolaris sorokiniana isolates determined by random-amplified polymorphic DNA (RAPD)

A. T. S. Matsumura, Prestes, A. M., Van Der Sand, S. T., and de Oliveira, A. M. R., Intraspecific variability of Bipolaris sorokiniana isolates determined by random-amplified polymorphic DNA (RAPD), vol. 1, pp. 350-358, 2002.

Isolates of Bipolaris sorokiniana were analyzed by random-amplified polymorphic DNA (RAPD) techniques to determine the amount of intraspecific genetic variability and to study host-pathogen interactions. Ten isolates originated from different regions of Brazil were examined. Plants of the wheat cultivars BR8, BH1146 (original host) and IAC-5 Maringá, classified as resistant, moderately resistant or susceptible to B. sorokiniana, respectively, were inoculated with these 10 isolates.

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