Rat

Therapeutic influence of intraperitoneal injection of Wharton's jelly-derived mesenchymal stem cells on oviduct function and fertility in rats with acute and chronic salpingitis

H. J. Luo, Xiao, X. M., Zhou, J., and Wei, W., Therapeutic influence of intraperitoneal injection of Wharton's jelly-derived mesenchymal stem cells on oviduct function and fertility in rats with acute and chronic salpingitis, vol. 14, pp. 3606-3617, 2015.

To study the effect of Wharton’s jelly-derived mesenchymal stem cells (WJMSCs) on the rat salpingitis model, 50 female Wistar rats were randomly divided into one control and five model groups. Mixed bacteria were injected into the oviducts of model groups. The treated acute and chronic groups received intraperitoneal injections of WJMSCs (1 x 106) once a week for three weeks.

Cytoskeleton changes of airway smooth muscle cells in juvenile rats with airway remodeling in asthma and the RhoA/ROCK signaling pathway mechanism

B. Wei, Shang, Y. X., Li, M., Jiang, J., and Zhang, H., Cytoskeleton changes of airway smooth muscle cells in juvenile rats with airway remodeling in asthma and the RhoA/ROCK signaling pathway mechanism, vol. 13, pp. 559-569, 2014.

This article aimed to investigate changes in the cytoskeleton of airway smooth muscle cells (ASMCs) in juvenile rats with airway remodeling in asthma. We further investigated the involvement of the RhoA/ROCK signaling pathway mechanism. Rat models of airway remodeling in asthma were established by antigen sensitization with ovalbumin for 2, 4, 6, and 8 weeks. The control group was treated with normal saline instead of ovalbumin. In the intervention group, after 8 weeks of culture, ASMCs were treated with the ROCK-specific inhibitor Y-27632.

The ratio of transforming growth factor-β1/bone morphogenetic protein-7 in the progression of the epithelial-mesenchymal transition contributes to rat liver fibrosis

W. R. Bi, Xu, G. T., Lv, L. X., and Yang, C. Q., The ratio of transforming growth factor-β1/bone morphogenetic protein-7 in the progression of the epithelial-mesenchymal transition contributes to rat liver fibrosis, vol. 13, pp. 1005-1014, 2014.

This study was designed to show whether rat liver epithelial cells could undergo epithelial-mesenchymal transition (EMT), thereby directly contributing to liver fibrosis. The role of the ratio of transforming growth factor-β1 (TGF-β1)/bone morphogenetic protein-7 (BMP-7) was evaluated in the progression of EMT or mesenchymal-epithelial transition. Primary rat liver epithelial cells were stimulated with different ratios of TGF-β1/BMP-7 and examined for evidence of transition to a mesenchymal or epithelial phenotype.

Relationship between renal injury and the antagonistic roles of angiotensin-converting enzyme (ACE) and ACE2

C. Ma, Xin, H., Jiang, X. - Y., Wang, Y. - X., and Zhang, Y. - S., Relationship between renal injury and the antagonistic roles of angiotensin-converting enzyme (ACE) and ACE2, vol. 13, pp. 2333-2342, 2014.

Angiotensin-converting enzyme 2 (ACE2), a newly discovered carboxypeptidase in the renin-angiotensin system (RAS), antagonizes ACE activity and plays an active role during tissue injury. Yet the mechanism of its action is not well known. Using a streptozotocin (STZ)-induced renal injury rat model, we investigated the relationship between renal injury and the antagonism between ACE and ACE2.

Evaluation of attenuated Salmonella choleraesuis-mediated inhibin recombinant DNA vaccine in rats

F. M. Hui, Meng, C. L., Guo, N. N., Yang, L. G., Shi, F. X., and Mao, D. G., Evaluation of attenuated Salmonella choleraesuis-mediated inhibin recombinant DNA vaccine in rats, vol. 13, pp. 6113-6125, 2014.

DNA vaccination has been studied intensively as a potential vaccine technology. We evaluated the effect of an attenuated Salmonella choleraesuis-mediated inhibin DNA vaccine in rats. First, 15 rats were treated with different doses of an inhibin vaccine to evaluate vaccine safety. Next, 30 rats were divided into 3 groups and injected intramuscularly with the inhibin vaccine two (T1) or three times (T2) or with control bacteria (Con) at 4-week intervals.

Changes in cyclic GMP level and phosphodiesterase activity during follicular development in the rat ovary

C. Q. Fu, Shi, F. X., Zhang, Z. H., Li, J. R., Huang, X. H., and Wang, Z. C., Changes in cyclic GMP level and phosphodiesterase activity during follicular development in the rat ovary, vol. 13, pp. 5919-5928, 2014.

Guanosine 3',5'-cyclic monophosphate (cGMP), as a second messenger, plays potential roles in ovarian functions. To elucidate the role of phosphodiesterase (PDE) in cGMP signaling during ovarian follicular development, the present study was conducted to investigate ovarian cGMP level and cGMP-PDE activity by radioimmunoassay (RIA) in postnatal rats, immature rats during gonadotropin-primed follicular development, ovulation and luteinization, adult rats during normal estrous cycle, and aged rats that spontaneously developed persistent estrus (PE).

Effects of acrylonitrile on the pathological morphology and apoptosis of neurons in rats

C. Z. Li, Tse, L. A., Zhou, Y. L., Fan, W., Li, M., Yu, I. T. S., and Jin, T. Y., Effects of acrylonitrile on the pathological morphology and apoptosis of neurons in rats, vol. 13, pp. 5795-5802, 2014.

This study aimed to evaluate the effects of acrylonitrile (ACN) on neuronal morphology and apoptosis in rats. An ACN solution was administered to Wistar rats by gavage at doses of 0, 5, 10, or 20 mg/kg, 5 days a week for 13 weeks. The morphology of neurons and the presence of apoptosis was examined by light and electron microscope, DNA electrophoresis, immunohistochemistry, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling.

Intermedin protects against myocardial ischemia-reperfusion injury in hyperlipidemia rats

S. M. Yang, Liu, J., and Li, C. X., Intermedin protects against myocardial ischemia-reperfusion injury in hyperlipidemia rats, vol. 13, pp. 8309-8319, 2014.

Hyperlipidemia is a well-established risk factor for the development of coronary atherosclerosis, while intermedin (IMD) has been identified as a novel calcitonin/calcitonin gene-related peptide family member involved in cardiovascular protection. However, whether IMD protects against hyperlipidemia-associated myocardial ischemia/reperfusion (MI/R) injury is unknown. We established a hyperlipidemia model using Sprague-Dawley rats, and created a MI/R condition by ligating the cardiac left circumflex artery.

Association of Hsp60 expression with damage to rat myocardial cells exposed to heat stress in vivo and in vitro

Y. F. Cheng, Sun, J. R., Chen, H. B., Abdelnasir, A., Tang, S., Kemper, N., Hartung, J., and Bao, E. D., Association of Hsp60 expression with damage to rat myocardial cells exposed to heat stress in vivo and in vitro, vol. 13, pp. 9371-9381, 2014.

To investigate the protective role of Hsp60 against stress damage and its role in the sudden death of stressed animals, changes in the levels of Hsp60 protein and hsp60 mRNA of myocardial cells in vivo and in vitro were studied. In addition, the relationship between Hsp60 expression and heat-induced damage was also studied. Rats were exposed to a temperature of 42° ± 1°C for 0, 20, 40, 60, 80, or 100 min. More than 50% of the rats died suddenly within 100 min.

Effect of the IGF-1/PTEN/Akt/FoxO signaling pathway in the duodenal mucosa of rats subjected to water immersion and restraint stress

P. Huang, Zhou, Z. R., Zheng, M. Q., and Shi, F. X., Effect of the IGF-1/PTEN/Akt/FoxO signaling pathway in the duodenal mucosa of rats subjected to water immersion and restraint stress, vol. 11, pp. 4775-4788, 2012.

The insulin growth factor 1/phosphatase and tensin homologue deleted on chromosome 10/Akt/forkhead box (IGF-1/PTEN/Akt/FoxO) signaling pathway reportedly exhibits gastroprotective effects by reducing water immersion and restraint stress (WRS)-induced gastric mucosal cell apoptosis. We examined the expression and localization of IGF-1, PTEN, Akt, and FoxO proteins, caspase-3 activity, and the number of apoptotic cells in the duodenal mucosa of rats subjected to WRS to confirm whether the IGF-1/PTEN/Akt/FoxO signaling pathway has a role in the duodenal mucosa.

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