Rats

Effect of nimodipine on rat spinal cord injury

Y. - F. Jia, Gao, H. - L., Ma, L. - J., and Li, J., “Effect of nimodipine on rat spinal cord injury”, vol. 14, pp. 1269-1276, 2015.

We evaluated the potentially protective effect of nimodipine on rat spinal cord injury. Sprague-Dawley rats received spinal cord injury, and were separated into nimodipine (N = 12) and saline groups (N = 12). Within 1 h of the injury, rats were treated intraperitoneally with nimodipine (1.0 mg/kg) or an equal amount of saline. Treatment was performed 3 times a day for 1 week. Operation BBB score and track experiment were used to measure the physical function of the hind legs 1 and 2 weeks after injury.

Association of heat shock protein 70 expression with rat myocardial cell damage during heat stress in vitro and in vivo

H. B. Chen, Zhang, X. C., Cheng, Y. F., Abdelnasir, A., Tang, S., Kemper, N., Hartung, J., and Bao, E. D., “Association of heat shock protein 70 expression with rat myocardial cell damage during heat stress in vitro and in vivo”, vol. 14, pp. 1994-2005, 2015.

To investigate the mechanism of sudden death as a result of stress-induced damage to heart tissue and myocardial cells and to investigate the cardioprotective role of Hsp70 during heat stress, the distribution and expression of Hsp70 was evaluated in the heart cells of heat-stressed rats in vivo and heat-stressed H9c2 cells in vitro.

Protective effect of ischemia preconditioning on ischemia-reperfusion injury in rat liver transplantation

Z. S. Qing, Zhang, X. S., Gao, C. C., Liu, W. D., Xia, T. F., Wu, K., and Pang, L. Q., “Protective effect of ischemia preconditioning on ischemia-reperfusion injury in rat liver transplantation”, vol. 14, pp. 3018-3025, 2015.

We explored the protective effect of ischemia preconditioning (IP) on ischemia-reperfusion injury in rat liver transplantation. An orthotopic liver transplantation model was utilized in the study. A total of 54 Sprague-Dawley rats were divided into a control group (group A, no liver transplantation), liver transplantation group (group B, heparin Ringer’s lactate solution was perfused via the portal vein before donor liver collection), and liver transplantation with IP group (group C, IP was performed for different time periods before donor liver collection).

Neuroprotective effect of ketamine on acute spinal cord injury in rats

S. H. Tang, Yu, J. G., Li, J. J., and Sun, J. Y., “Neuroprotective effect of ketamine on acute spinal cord injury in rats”, vol. 14, pp. 3551-3556, 2015.

The aim of this study was to investigate the neuroprotective effects of ketamine during acute spinal cord injury in rats. Sprague Dawley (SD) rats (N = 70) were randomly divided into three groups: sham-operated (N = 10), control (N = 30), and treatment (N = 30) groups. The moderate spinal cord injury model was established. After injury, the sham-operated group received no drug, the treatment group received intraperitoneal ketamine injections, and the control group received intraperitoneal normal saline injections.

Hypoxia-inducible factor-1α expression in the different stages of rat thromboangiitis obliterans

T. He, Qu, B. H., Wang, D. L., and Hu, M., “Hypoxia-inducible factor-1α expression in the different stages of rat thromboangiitis obliterans”, vol. 14, pp. 6715-6722, 2015.

We investigated the expression and effects of hypoxia-inducible factor-1α (HIF-1α) in rat thromboangiitis obliterans (TO). Rats were divided into sham and model groups. The model group was further divided into groups based on observation duration. Lauric acid was injected below an artery clamp to simulate TO in the model group; saline was used in the sham group. Clamps were removed 15 min after injection in both groups, and physiological changes were observed at different times (gross observation and hematoxylin and eosin staining).

Effects of thrombin on the secondary cerebral injury of perihematomal tissues of rats after intracerebral hemorrhage

C. M. Liu, Shi, B. Z., and Zhou, J. S., “Effects of thrombin on the secondary cerebral injury of perihematomal tissues of rats after intracerebral hemorrhage”, vol. 13, pp. 4617-4626, 2014.

This study aimed to investigate the effects of thrombin released in hematoma after intracerebral hemorrhage (ICH) on the cerebral injury of perihematomal tissues and to evaluate the protection effect of hirudin on the cerebral injury after ICH. We used the autologous uncoagulated blood injection method to prepare the ICH rat model, and all rats were randomly divided into a normal group, an ICH group, or a hirudin group. At different time points, rat heads were cut to harvest brain sections.

Effects of different doses of Savda Munziq on myocardial ischemia-reperfusion injury in rats with abnormal Savda syndrome

A. Maimaitiaili, Shabiti, A., Abudureheman, M., Musha, Z., Jun, Q., Maimaitiaili, G., Aibibula, A., and Upur, H., “Effects of different doses of Savda Munziq on myocardial ischemia-reperfusion injury in rats with abnormal Savda syndrome”, vol. 13, pp. 4729-4735, 2014.

To investigate the effects of different doses of abnormal Savda Munziq on myocardial ischemia-reperfusion injury (MI/RI) in rats with the abnormal Savda syndrome, 50 abnormal Savda animal models were randomly divided into a control group, a model group, a high-dose group, a middle-dose group, and a low-dose group, with each group containing 10 rats. The enzyme-linked immunosorbent assay was used to detect the serum myocardial enzyme and troponin levels, and hematoxylin and eosin (HE) staining was used to observe changes of the myocardial tissues in the different groups.

PPAR-γ agonist pioglitazone affects rat gouty arthritis by regulating cytokines

R. - C. Wang and Jiang, D. - M., “PPAR-γ agonist pioglitazone affects rat gouty arthritis by regulating cytokines”, vol. 13, pp. 6577-6581, 2014.

The objective was to study peroxisome proliferator-activated receptor gamma (PPARγ) agonist pioglitazone regulation effect and its mechanism of expression of cytokines on acute gouty arthritis synovial in rats. Rats with unilateral ankle were injected with artificial monosodium urate (MSU) crystals to make the acute gouty arthritis model.

Transient increase in IL-1β, IL-6 and TNF-α gene expression in rat liver exposed to gold nanoparticles

H. A. Khan, Abdelhalim, M. A. K., Alhomida, A. S., and Ayed, M. S. Al, “Transient increase in IL-1β, IL-6 and TNF-α gene expression in rat liver exposed to gold nanoparticles”, vol. 12. pp. 5851-5857, 2013.

Most studies have used in vitro systems to test inflammatory responses of nanoparticles; these may not reflect the real biological response of body organs. In fact, certain nanoparticles have provoked opposite effects under in vitro and in vivo conditions. Current understanding of the biocompatibility of gold nanoparticles is controversial. We studied the acute (1 day) and sub-chronic (5 days) effects of gold nanoparticles (10 and 50 nm in diameter) on expression of interleukin-1 beta (IL-1β), IL-6 and tumor necrosis factor alpha (TNF-α) in rat liver.