Recombinant expression

Expression and antibody generation of the cancer-testis antigen, BIOT2-S

J. - Y. Wang, Cao, M., Guo, M. - R., Li, S., Yang, X. - F., Wang., M., Fang, J., and Zhao, J., Expression and antibody generation of the cancer-testis antigen, BIOT2-S, vol. 14, pp. 8685-8693, 2015.

Biot2-S is a mouse cancer-testis antigen gene that was identified using the cross-reactive serological analysis of recombinant cDNA expression libraries (SEREX) technique in the State Key Laboratory of Biotherapy, West China Hospital, Sichuan University. To express BIOT2-S and generate its antibody for further investigation, the Biot2-S prokaryotic recombinant expression vector Biot2-S/pGEX6P-1 was constructed with Escherichia coli DH5α as a cloning vector, and BIOT2-S was expressed in E. coli Rosetta (DE3).

Subscribe to Recombinant expression