Characterization and heterologous expression of isocitrate lyase of Paracoccidioides brasiliensis
, “Characterization and heterologous expression of isocitrate lyase of Paracoccidioides brasiliensis”, vol. 11. pp. 4297-4298, 2012.
Recombinant protein
Molecular cloning of HSP70 in Mycoplasma ovipneumoniae and comparison with that of other mycoplasmas
, “Molecular cloning of HSP70 in Mycoplasma ovipneumoniae and comparison with that of other mycoplasmas”, vol. 10, pp. 834-848, 2011.
Mycoplasma ovipneumoniae, a bacterial species that specifically affects ovine and goat, is the cause of ovine infectious pleuropneumonia. We cloned, sequenced and analyzed heat shock protein 70 (HSP70) (dnaK) gene of M. ovipneumoniae. The full length open reading frame of the M. ovipneumoniae HSP70 gene consists of 1812 nucleotides, with a G+C content of 34.16%, encoding 604 amino acids.
Analysis of protein expression and a new prokaryotic expression system for goat (Capra hircus) spermadhesin Bdh-2 cDNA
, “Analysis of protein expression and a new prokaryotic expression system for goat (Capra hircus) spermadhesin Bdh-2 cDNA”, vol. 8, pp. 1147-1157, 2009.
Low purification efficiency and incomplete characterization of male goat (buck) spermadhesins (Bdhs) prompted us to develop an effective system to produce recombinant Bdhs (rBdhs). Bdh-2 cDNA was inserted into a prokaryotic expression plasmid, pTrcHis TOPO. The pTrcHis-Bdh-2 system was constructed to produce a His6 fusion protein in Escherichia coli Top10 cells. Recombinant clones were selected by growth in ampicillin-enriched medium, PCR amplification and nucleotide sequencing.
