Resistance

Identification of aac(2')-I Type b aminoglycoside-modifying enzyme genes in resistant Acinetobacter baumannii

T. Lin, Tang, C. G., Li, Q. H., Ji, J., Ge, H. Y., Zhang, X. Y., and Sun, H. P., Identification of aac(2')-I Type b aminoglycoside-modifying enzyme genes in resistant Acinetobacter baumannii, vol. 14, pp. 1828-1835, 2015.

The aim of this study was to investigate the mechanism underlying the drug resistance of Acinetobacter baumannii toward aminoglycosides. A total of 32 A. baumannii strains were identified by molecular identification and subsequently isolated. The isolates were then amplified by polymerase chain reaction to analyze the 9 aminoglycoside-modifying enzyme genes and 7 16S rRNA methylase genes. Five types of aminoglycoside-modifying enzyme genes and 1 type of 16S rRNA methylase gene were detected in the 32 drug-resistant A. baumannii strains.

Relevance of E-cadherin expression to EGFR-TKI molecular targeted therapy sensitivity/resistance and its clinical significance

R. C. Xing, Zheng, J., Zheng, W. H., Qin, Z. P., Liu, W., and Yao, R. C., Relevance of E-cadherin expression to EGFR-TKI molecular targeted therapy sensitivity/resistance and its clinical significance, vol. 14, pp. 5785-5792, 2015.

We examined the effect of E-cadherin expression on epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) molecular targeted therapy sensitivity/resistance. We treated MCF-7, MDA-MB-231, T24, SiHa, H460, SK-HEP-1, MHCC97-H, and THP-1 cells with the EGFR-TKIs PD153035 and gefitinib, and then tested the drug-resistance and sensitivity using the MTT method, calculated IC50 values for each cell line, and compared the results to E-cadherin content.

Detection of drug-resistance mechanism of Pseudomonas aeruginosa developing from a sensitive strain to a persister during carbapenem treatment

J. L. Shen and Fang, Y. P., Detection of drug-resistance mechanism of Pseudomonas aeruginosa developing from a sensitive strain to a persister during carbapenem treatment, vol. 14, pp. 6723-6732, 2015.

We explored the mechanism of the development from sensitivity to resistance to carbapenem in Pseudomonas aeruginosa. Two P. aeruginosa strains were collected during treatment with carbapenem. Strain homology was investigated using pulsed-field gel electrophoresis. Porin oprD2 expression was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The minimum inhibitory concentrations (MICs) of imipenem and meropenem with or without MC207110 were determined using the agar dilution method.

Comparative analysis of mucosal immunity to Mycoplasma hyopneumoniae in Jiangquhai porcine lean strain and DLY piglets

L. Z. Hua, Wu, Y. Z., Bai, F. F., William, K. K., Feng, Z. X., Liu, M. J., Yao, J. T., Zhang, X., and Shao, G. Q., Comparative analysis of mucosal immunity to Mycoplasma hyopneumoniae in Jiangquhai porcine lean strain and DLY piglets, vol. 13, pp. 5199-5206, 2014.

The Jiangquhai porcine lean strain (JQHPL) is a new pork meat-type strain that has been developed in recent years from the parent lines Duroc, Fengjing, and Jiangquhai pigs (DurocxFengjing pigxJiangquhai pig). Enzootic pneumonia (EP) in pigs induced by Mycoplasma hyopneumoniae (M. hyopneumoniae) is a chronic respiratory disease of pigs, generating high economic losses in the swine industry. Here, we investigated the degree of resistance to M.

Reaction of common bean lines and aggressiveness of Sclerotinia sclerotiorum isolates

P. H. Silva, Santos, J. B., Lima, I. A., Lara, L. A. C., and Alves, F. C., Reaction of common bean lines and aggressiveness of Sclerotinia sclerotiorum isolates, vol. 13, pp. 9138-9151, 2014.

The aims of this study were to evaluate the reaction of common bean lines to white mold, the aggressiveness of different Sclerotinia sclerotiorum isolates from various common bean production areas in Brazil, and comparison of the diallel and GGE (genotype main effect plus genotype-by-environment interaction) biplot analysis procedures via study of the line-by-isolate interaction. Eleven common bean (Phaseolus vulgaris) lines derived from 3 backcross populations were used.

Association of AFLP and SCAR markers with common leafspot resistance in autotetraploid alfalfa (Medicago sativa)

Y. Wang, Bi, B., Yuan, Q. H., Li, X. L., and Gao, J. M., Association of AFLP and SCAR markers with common leafspot resistance in autotetraploid alfalfa (Medicago sativa), vol. 11, pp. 606-616, 2012.

To identify amplified fragment length polymorphism (AFLP) markers associated with resistance or susceptibility of alfalfa to common leafspot (CLS) caused by the fungus Pseudopeziza medicaginis (Dermateaceae), bulked segregant analysis was conducted based on an F1(M × M) population of 93 plants and a BC1S population of 91 plants. Three AFLP markers, ACTCAAR206, TAGCACR185, and GGACTAS264, were found to be associated with CLS resistance or susceptibility.

RAPD identification of Varroa destructor genotypes in Brazil and other regions of the Americas

J. C. V. Guerra, Jr., Issa, M. R. C., Carneiro, F. E., Strapazzon, R., and Moretto, G., RAPD identification of Varroa destructor genotypes in Brazil and other regions of the Americas, vol. 9, pp. 303-308, 2010.

The mite Varroa destructor is the main pest causing damage to apiculture worldwide. In Brazil and other parts of the world, where bees of African origin and their hybrids predominate, the bees can survive these mites without treatment. Studies have shown a correlation between the various genotypes of the mite and its fertility in different geographical regions. Information about mite genotype could be helpful in understanding the diverse effects and relationships of the mite with bees in different regions of the world.

Differential structure of the intronic promoter of the Bombyx mori A3 actin gene correlated with silkworm sensitivity/resistance to nucleopolyhedrovirus

J. Tavares, Bravo, J. P., Gimenes, F., Neto, Q. A. L., Fiorini, A., and Fernandez, M. A., Differential structure of the intronic promoter of the Bombyx mori A3 actin gene correlated with silkworm sensitivity/resistance to nucleopolyhedrovirus, vol. 10, pp. 471-481, 2011.

Previous reports demonstrated that actin is necessary for nucleocapsid transport and viral gene expression during nucleopolyhedrovirus infection of Bombyx mori. The first intron of B. mori A3 actin contains a cryptic promoter that drives expression of a rare isoform. We detected differences in the size and nucleotide composition of the first intron of the A3 actin gene from B. mori strain C24A, which is more resistant to nucleopolyhedrovirus than the M11A strain (22 and 95% lethality, respectively).

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