Retrotransposons

Development of retrotransposon-based markers IRAP and REMAP for cassava (Manihot esculenta)

B. C. Kuhn, Mangolin, C. A., Souto, E. R., Vicient, C. M., Machado, M. F. P. S., Kuhn, B. C., Mangolin, C. A., Souto, E. R., Vicient, C. M., Machado, M. F. P. S., Kuhn, B. C., Mangolin, C. A., Souto, E. R., Vicient, C. M., and Machado, M. F. P. S., “Development of retrotransposon-based markers IRAP and REMAP for cassava (Manihot esculenta)”, vol. 15, p. -, 2016.

Retrotransposons are abundant in the genomes of plants. In the present study, inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) markers were developed for the cassava genome (Manihot esculenta Crantz). Four cassava cultivars (Fécula Branca, IPR-União, Olho Junto, and Tamboara, two samples per cultivar) were used to obtain IRAP and REMAP fingerprints. Twelve designed primers were amplified alone and in combinations.

Genetic bottlenecks in Turkish okra germplasm and utility of iPBS retrotransposon markers for genetic diversity assessment

M. Yildiz, Koçak, M., and Baloch, F. S., “Genetic bottlenecks in Turkish okra germplasm and utility of iPBS retrotransposon markers for genetic diversity assessment”, vol. 14, pp. 10588-10602, 2015.

Lack of requisite genetic variation in Turkish okra has necessitated the use of different types of markers for estimating the genetic diversity and identifying the source of variation. Transposable elements, present abundantly in plant genomes, generate genomic diversity through their replication and are thus an excellent source of molecular markers. We hypothesized that inter-primer binding site (iPBS)-retrotransposons could be the source of variation because of their genome plasticity nature.

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