RFLP-PCR

Analysis of α1 and α2 globin genes among patients with hemoglobin Adana in Malaysia

T. Y. Lee, Lai, M. I., Ismail, P., Ramachandran, V., Tan, J. A. M. A., Teh, L. K., Othman, R., Hussein, N. H., George, E., Lee, T. Y., Lai, M. I., Ismail, P., Ramachandran, V., Tan, J. A. M. A., Teh, L. K., Othman, R., Hussein, N. H., George, E., Lee, T. Y., Lai, M. I., Ismail, P., Ramachandran, V., Tan, J. A. M. A., Teh, L. K., Othman, R., Hussein, N. H., and George, E., Analysis of α1 and α2 globin genes among patients with hemoglobin Adana in Malaysia, vol. 15, p. -, 2016.

Hemoglobin (Hb) Adana [HBA2: c179G>A (or HBA1); p.Gly60Asp] is a non-deletional α-thalassemia variant found in Malaysia. An improvement in the molecular techniques in recent years has made identification of Hb Adana much easier. For this study, a total of 26 Hb Adana α-thalassemia intermedia and 10 Hb Adana trait blood samples were collected from patients. Common deletional and non-deletional α-thalassemia genotypes were determined using multiplex gap polymerase chain reaction (PCR) and multiplex ARMS PCR techniques.

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