RNA

Source of bacterial RNA in chronic otitis media with effusion

P. - Z. Li, Cheng, L., and Qiu, M. - L., Source of bacterial RNA in chronic otitis media with effusion, vol. 13, pp. 6093-6098, 2014.

The purpose of this study was to investigate whether the bacterial RNA detected by polymerase chain reaction (PCR) and reverse transcription (RT)-PCR methods in middle ear effusion (MEE) for pediatric chronic otitis media with effusion (OME) originated from live bacteria. Degradation of RNA was observed by spectroscopic analysis; we also investigated the effect of MEE on the digestive activity of RNase. The optical density of RNA solution was stable within 3 h. MEE could not degrade the RNA, while RNase could rapidly digest the RNA.

Analysis of blood processing conditions to obtain high-quality total RNA from human leukocyte concentrate

I. A. G. Pérez, Santana, S. P., Argudin, T. D., and Gardon, D. O. P., Analysis of blood processing conditions to obtain high-quality total RNA from human leukocyte concentrate, vol. 6, pp. 298-307, 2007.

Blood samples are used as a biological source to discover biomarkers of hematological and non-hematological disorders. The present study shows the impact of different experimental conditions associated with cell lysis buffer, TRI-reagent protocol and blood cell storage buffer and their correlation with the quantity, quality and Adrenomedullin gene expression levels of total RNA when RT-PCR technique is used. A leukocyte cell bank protocol is also proposed for further mRNA expression analysis using RNAlater as storage buffer.

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