RNA extraction

Comparison of two methods for RNA extraction from the nucleus pulposus of intervertebral discs

M. F. Gan, Yang, H. L., Qian, J. L., Wu, C. S., Yuan, C. X., Li, X. F., Zou, J., Gan, M. F., Yang, H. L., Qian, J. L., Wu, C. S., Yuan, C. X., Li, X. F., and Zou, J., Comparison of two methods for RNA extraction from the nucleus pulposus of intervertebral discs, vol. 15, p. -, 2016.

RNA extraction from the nucleus pulposus of intervertebral discs has been extensively used in orthopedic studies. We compared two methods for extracting RNA from the nucleus pulposus: liquid nitrogen grinding and enzyme digestion. The RNA was detected by agarose gel electrophoresis, and the purity was evaluated by absorbance ratio using a spectrophotometer. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression was assayed by reverse transcription-polymerase chain reaction (RT-PCR). Thirty human lumbar intervertebral discs were used in this study.

Integrity of prokaryotic mRNA isolated from complex samples for in vivo bacterial transcriptome analysis

A. B. Ferreira-Machado, Freitas, M. C. R., Saji, G. R. Q., Rezende, A. B., Almeida, P. E., Cesar, D. E., Resende, J. A., Nicólas, M. F., Silva, V. L., and Diniz, C. G., Integrity of prokaryotic mRNA isolated from complex samples for in vivo bacterial transcriptome analysis, vol. 14, pp. 14752-14759, 2015.

Even though several in vitro studies have focused on bacterial biology, the extent of such knowledge is not complete when considering an actual infection. As culture-independent microbiology methods such as high-throughput sequencing became available, important aspects of host-bacterium interactions will be elucidated. Based on microbiological relevance, we considered Bacteroides fragilis in a murine experimental infection as a model system to evaluate the in vivo bacterial transcriptome in host exudates.

Effect of DNase treatment on RNA extraction from preimplantation murine embryos

J. Norhazlin, Nor-Ashikin, M. N. K., Hoh, B. P., Kadir, S. H. Sheikh Abd, Norita, S., Mohd-Fazirul, M., Wan-Hafizah, W. J., Razif, D., Rajikin, M. H., and Abdullah, B., Effect of DNase treatment on RNA extraction from preimplantation murine embryos, vol. 14, pp. 10172-10184, 2015.

The quality of RNA is crucial when performing microarray experiments. This is particularly important when dealing with preimplantation embryos, from which a minimum yield of RNA of good quality can be produced. We report the optimization of several RNA extraction methods applied to preimplantation embryos at different stages of development. The quality of the samples was confirmed using a microarray and reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) analysis.

Subscribe to RNA extraction