Root

Efficient method of protein extraction from Theobroma cacao L. roots for two-dimensional gel electrophoresis and mass spectrometry analyses

F. Z. Bertolde, Almeida, A. - A. F., Silva, F. A. C., Oliveira, T. M., and Pirovani, C. P., “Efficient method of protein extraction from Theobroma cacao L. roots for two-dimensional gel electrophoresis and mass spectrometry analyses”, vol. 13, pp. 5036-5047, 2014.

Theobroma cacao is a woody and recalcitrant plant with a very high level of interfering compounds. Standard protocols for protein extraction were proposed for various types of samples, but the presence of interfering compounds in many samples prevented the isolation of proteins suitable for two-dimensional gel electrophoresis (2-DE). An efficient method to extract root proteins for 2-DE was established to overcome these problems.

Genotypic variability for root/shoot parameters under water stress in some advanced lines of cotton (Gossypium hirsutum L.)

M. Riaz, Farooq, J., Sakhawat, G., Mahmood, A., Sadiq, M. A., and Yaseen, M., “Genotypic variability for root/shoot parameters under water stress in some advanced lines of cotton (Gossypium hirsutum L.)”, vol. 12, pp. 552-561, 2013.

Research pertaining to genetic variability parameters, heritability, and genotypic, phenotypic, simple, and environmental correlations for various seedling traits in five elite advanced cotton (Gossypium hirsutum L.) lines (FH-113, FH-114, FH-941, FH-942, and FH-2015) and one check (CIM-496) was carried out during October and November 2010 under greenhouse conditions at the Cotton Research Institute (Faisalabad, Pakistan). Material was raised in plastic tubes with a randomized complete block design replicated three times.

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