RT-PCR

Network spatio-temporal analysis predicts disease stage-related genes and pathways in renal cell carcinoma

X. H. Li, Yang, C. Z., Wang, J., Li, X. H., Yang, C. Z., and Wang, J., Network spatio-temporal analysis predicts disease stage-related genes and pathways in renal cell carcinoma, vol. 15, p. -, 2016.

The purpose of this study was to screen the key genes and pathways of renal cell carcinoma (RCC) and lay the foundation for its diagnosis and therapy. Microarray data of normal subjects and RCC patients at different stages of disease were used to screen differentially expressed genes (DEGs). Based on the DEGs in the four disease stages, four co-expression networks were constructed using the Empirical Bayes method and hub genes were obtained by centrality analysis. The enriched pathways of the DEGs and the mutual hub genes in the cluster of each disease stage were investigated.

Rapamycin enhances IFN-γ and IL-4 production in co-culture of gδ T and dendritic cells from mice with lipopolysaccharide-induced acute lung injury

X. L. Chen, Li, J., Xu, G. G., Li, H. X., Guo, J., Chen, X. L., Li, J., Xu, G. G., Li, H. X., Guo, J., Chen, X. L., Li, J., Xu, G. G., Li, H. X., and Guo, J., Rapamycin enhances IFN-γ and IL-4 production in co-culture of gδ T and dendritic cells from mice with lipopolysaccharide-induced acute lung injury, vol. 15, p. -, 2016.

This study aimed to study the role of rapamycin (RAPA) in modulating the interaction between gδ T cells and dendritic cells (DCs) in a lipopolysaccharide (LPS)-induced acute lung injury mouse model. Mice were injected with LPS to establish the acute lung injury model or LPS + RAPA to assess the role of RAPA in modulating cell interactions. Mice were injected with PBS or RAPA alone as controls. gδ T cells and DCs were isolated from all mice and assessed by flow cytometry and fluorescence microscopy.

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