RT-qPCR

RT-qPCR assay on the vitamin D receptor gene in type 2 diabetes and hypertension patients in Turkey

H. C. Vural and Maltas, E., RT-qPCR assay on the vitamin D receptor gene in type 2 diabetes and hypertension patients in Turkey, vol. 11, pp. 582-590, 2012.

RT-qPCR was used to analyze the vitamin D receptor (VDR) gene TaqI polymorphism in 100 Turkish patients with type 2 diabetes mellitus (T2DM) and hypertension compared with 100 healthy subjects, to determine whether VDR could be considered as one of the susceptibility genes for T2DM and hypertension. Genotyping was done with PCR, followed by melting curve analysis with specific fluorescent hybridization probes. The results showed that distributions for TT, Tt and tt genotypes were 51, 46 and 3% in the patient group, and 35, 49 and 16% in the control group, respectively.

Expression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae)

M. A. Lopes, Júnior, B. T. Hora, Júnior, B. T. Hora, Dias, C. V., Santos, G. C., Gramacho, K. P., Cascardo, J. C. M., Gesteira, A. S., and Micheli, F., Expression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae), vol. 9, pp. 1279-1297, 2010.

Cacao (Theobroma cacao) is one of the most important tropical crops; however, production is threatened by numerous pathogens, including the hemibiotrophic fungus Moniliophthora perniciosa, which causes witches’ broom disease. To understand the mechanisms that lead to the development of this disease in cacao, we focused our attention on cacao transcription factors (TFs), which act as master regulators of cellular processes and are important for the fine-tuning of plant defense responses.

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